ArticlePloS one2013
Characterization of two 20kDa-cement protein (cp20k) homologues in Amphibalanus amphitrite.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Bioinspired bioadhesion: translating nature's adhesive strategies into regenerative medicine.Med-X · 2026Review
- Exogenous adenosine promotes barnacle (Amphibalanus amphitrite) cyprid settlement through molecular signaling and improved adhesive mechanics.Communications biology · 2025Article
- Aboral cell types ofScience advances · 2025Article
- Adhesive Materials Inspired by Barnacle Underwater Adhesion: Biological Principles and Biomimetic Designs.Frontiers in bioengineering and biotechnology · 2022Review
- An essential role of disulfide bonds for the hierarchical self-assembly and underwater affinity of CP20-derived peptides.Frontiers in bioengineering and biotechnology · 2022Article
- Proteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck BarnacleInternational journal of molecular sciences · 2021Article
- The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle,International journal of molecular sciences · 2020Article
- Insights into the Synthesis, Secretion and Curing of Barnacle Cyprid Adhesive via Transcriptomic and Proteomic Analyses of the Cement Gland.Marine drugs · 2020Article
- Adhesion of acorn barnacles on surface-active borate glasses.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Sequence basis of Barnacle Cement Nanostructure is Defined by Proteins with Silk Homology.Scientific reports · 2016Article
- Secretory locations of SIPC in Amphibalanus amphitrite cyprids and a novel function of SIPC in biomineralization.Scientific reports · 2016Article
- Structural characterization of tick cement cones collected from in vivo and artificial membrane blood-fed Lone Star ticks (Amblyomma americanum).Ticks and tick-borne diseases · 2016Article
- Molt-dependent transcriptomic analysis of cement proteins in the barnacle Amphibalanus amphitrite.BMC genomics · 2015Article
- p38 MAPK regulates PKAα and CUB-serine protease in Amphibalanus amphitrite cyprids.Scientific reports · 2015Article
- The chemistry of stalked barnacle adhesive (Lepas anatifera).Interface focus · 2015Article
- Sea star tenacity mediated by a protein that fragments, then aggregates.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Adhesive proteins of stalked and acorn barnacles display homology with low sequence similarities.PloS one · 2014Article
- Quantitative proteomics study of larval settlement in the Barnacle Balanus amphitrite.PloS one · 2014Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The barnacle, Amphibalanus amphitrite, is a common marine fouling organism. Understanding the mechanism of barnacle adhesion will be helpful in resolving the fouling problem. Barnacle cement is thought to play a key role in barnacle attachment. Although several adult barnacle cement proteins have been identified in Megabalanus rosa, little is known about their function in barnacle settlement. In this study, two homologous 20k-cement proteins (cp20k) in Amphibalanus amphitrite, named Bamcp20k-1 and Bamcp20k-2, were characterized. The two homologues share primary sequence structure with proteins from other species including Megabalanus rosa and Fistulobalanus albicostatus. The conserved structure included repeated Cys domains and abundant charged amino acids, such as histidine. In this study we demonstrated that Bamcp20k-1 localized at the α secretory cells in the cyprid cement gland, while Bamcp20k-2 localized to the β secretory cells. The differential localizations suggest differential regulation for secretion from the secretory cells. Both Bamcp20k-1 and Bamcp20k-2 from cyprids dissolved in PBS. However, adult Bamcp20k-2, which was dominant in the basal shell of adult barnacles, was largely insoluble in PBS. Solubility increased in the presence of the reducing reagent Dithiothreitol (DTT), suggesting that the formation of disulfide bonds plays a role in Bamcp20k-2 function. In comparison, Bamcp20k-1, which was enriched in soft tissue, could not be easily detected in the shell and base by Western blot and easily dissolved in PBS. These differential solubilities and localizations indicate that Bamcp20k-1 and Bamcp20k-2 have distinct functions in barnacle cementing.
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