ArticleProceedings of the National Academy of Sciences of the United States of America2013
Hybrid DNA virus in Chinese patients with seronegative hepatitis discovered by deep sequencing.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 59 citations in OpenAlex.
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- Summary of taxonomy changes ratified by the International Committee on Taxonomy of Viruses (ICTV) from the Fungal and Protist Viruses Subcommittee, 2025.The Journal of general virology · 2025Article
- Analysis of the distribution characteristics and influencing factors of viruses carried by atmospheric PMFrontiers in public health · 2025Article
- Expanding known viral diversity in plants: virome of 161 species alongside an ancient canal.Environmental microbiome · 2022Article
- Discovery and Surveillance of Tick-Borne Pathogens.Journal of medical entomology · 2021Article
- Contamination Issue in Viral Metagenomics: Problems, Solutions, and Clinical Perspectives.Frontiers in microbiology · 2021Review
- Sequencing facility and DNA source associated patterns of virus-mappable reads in whole-genome sequencing data.Genomics · 2021Article
- Performance of Metagenomic Next-Generation Sequencing for the Diagnosis of Viral Meningoencephalitis in a Resource-Limited Setting.Open forum infectious diseases · 2020Article
- Detection and Genetic Characterization of Viruses Present in Free-Ranging Snow Leopards Using Next-Generation Sequencing.Frontiers in veterinary science · 2020Article
- Human oncogenic viruses: nature and discovery.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Article
- Tumour virology in the era of high-throughput genomics.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Viral metagenomics applied to blood donors and recipients at high risk for blood-borne infections.Blood transfusion = Trasfusione del sangue · 2016Article
- Blood-Borne Pathogens: A Canadian Blood Services Centre for Innovation Symposium.Transfusion medicine reviews · 2016Article
- Identification of Known and Novel Recurrent Viral Sequences in Data from Multiple Patients and Multiple Cancers.Viruses · 2016Article
- Viral metagenomics and blood safety.Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine · 2016Review
- A Novel Astrovirus-Like RNA Virus Detected in Human Stool.Virus evolution · 2016Article
- Multiple layers of chimerism in a single-stranded DNA virus discovered by deep sequencing.Genome biology and evolution · 2015Article
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Seronegative hepatitis--non-A, non-B, non-C, non-D, non-E hepatitis--is poorly characterized but strongly associated with serious complications. We collected 92 sera specimens from patients with non-A-E hepatitis in Chongqing, China between 1999 and 2007. Ten sera pools were screened by Solexa deep sequencing. We discovered a 3,780-bp contig present in all 10 pools that yielded BLASTx E scores of 7e-05-0.008 against parvoviruses. The complete sequence of the in silico-assembled 3,780-bp contig was confirmed by gene amplification of overlapping regions over almost the entire genome, and the virus was provisionally designated NIH-CQV. Further analysis revealed that the contig was composed of two major ORFs. By protein BLAST, ORF1 and ORF2 were most homologous to the replication-associated protein of bat circovirus and the capsid protein of porcine parvovirus, respectively. Phylogenetic analysis indicated that NIH-CQV is located at the interface of Parvoviridae and Circoviridae. Prevalence of NIH-CQV in patients was determined by quantitative PCR. Sixty-three of 90 patient samples (70%) were positive, but all those from 45 healthy controls were negative. Average virus titer in the patient specimens was 1.05 e4 copies/µL. Specific antibodies against NIH-CQV were sought by immunoblotting. Eighty-four percent of patients were positive for IgG, and 31% were positive for IgM; in contrast, 78% of healthy controls were positive for IgG, but all were negative for IgM. Although more work is needed to determine the etiologic role of NIH-CQV in human disease, our data indicate that a parvovirus-like virus is highly prevalent in a cohort of patients with non-A-E hepatitis.
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