ArticleInfection and immunity2013
FeoB-mediated uptake of iron by Francisella tularensis.
Article in Infection and immunity, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Functional Characterization of FeoAB in Iron Acquisition and Pathogenicity in Riemerella anatipestifer.Microbiology spectrum · 2023Article
- A general protocol for the expression and purification of the intact transmembrane transporter FeoB.Biochimica et biophysica acta. Biomembranes · 2022Article
- Vanadate inhibits Feo-mediated iron transport in Vibrio cholerae.Metallomics : integrated biometal science · 2021Article
- The FeoC [4Fe-4S] Cluster Is Redox-Active and Rapidly Oxygen-Sensitive.Biochemistry · 2019Article
- Citryl Ornithine Is an Intermediate in a Three-Step Biosynthetic Pathway for Rhizoferrin inACS chemical biology · 2019Article
- Toward a mechanistic understanding of Feo-mediated ferrous iron uptake.Metallomics : integrated biometal science · 2018Review
- Zinc Acquisition Mechanisms Differ between Environmental and Virulent Francisella Species.Journal of bacteriology · 2018Article
- The Ability to Acquire Iron Is Inversely Related to Virulence and the Protective Efficacy ofFrontiers in microbiology · 2018Article
- Differential Growth ofFrontiers in microbiology · 2017Article
- Iron and Virulence inFrontiers in cellular and infection microbiology · 2017Review
- The Bradyrhizobium japonicum Ferrous Iron Transporter FeoAB Is Required for Ferric Iron Utilization in Free Living Aerobic Cells and for Symbiosis.The Journal of biological chemistry · 2016Article
- Two parallel pathways for ferric and ferrous iron acquisition support growth and virulence of the intracellular pathogen Francisella tularensis Schu S4.MicrobiologyOpen · 2016Article
- Gallium Potentiates the Antibacterial Effect of Gentamicin against Francisella tularensis.Antimicrobial agents and chemotherapy · 2016Article
- Successful protection against tularemia in C57BL/6 mice is correlated with expansion of Francisella tularensis-specific effector T cells.Clinical and vaccine immunology : CVI · 2015Article
- Interactions of Francisella tularensis with Alveolar Type II Epithelial Cells and the Murine Respiratory Epithelium.PloS one · 2015Article
- Mechanisms of heme utilization by Francisella tularensis.PloS one · 2015Article
- Article
- Shared and distinct mechanisms of iron acquisition by bacterial and fungal pathogens of humans.Frontiers in cellular and infection microbiology · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Francisella tularensis, the bacterial cause of tularemia, infects the liver and replicates in hepatocytes in vivo and in vitro. However, the factors that govern adaptation of F. tularensis to the intrahepatocytic niche have not been identified. Using cDNA microarrays, we determined the transcriptional profile of the live vaccine strain (LVS) of F. tularensis grown in the FL83B murine hepatocytic cell line compared to that of F. tularensis cultured in broth. The fslC gene of the fsl operon was the most highly upregulated. Deletion of fslC eliminated the ability of the LVS to produce siderophore, which is involved in uptake of ferric iron, but it did not impair its growth in hepatocytes, A549 epithelial cells, or macrophages. Therefore, we sought an alternative means by which F. tularensis might obtain iron. Deletion of feoB, which encodes a putative ferrous iron transporter, retarded replication of the LVS in iron-restricted media, reduced its growth in hepatocytic and epithelial cells, and impaired its acquisition of iron. Survival of mice infected intradermally with a lethal dose of the LVS was slightly improved by deletion of fslC but was not altered by loss of feoB. However, the ΔfeoB mutant showed diminished ability to colonize the lungs, liver, and spleen of mice that received sublethal inocula. Thus, FeoB represents a previously unidentified mechanism for uptake of iron by F. tularensis. Moreover, failure to produce a mutant strain lacking both feoB and fslC suggests that FeoB and the proteins of the fsl operon are the only major means by which F. tularensis acquires iron.
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Registered trials
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