ArticleThe American journal of tropical medicine and hygiene2013
Monoclonal antibody-based immunofluorescence microscopy for the rapid identification of Burkholderia pseudomallei in clinical specimens.
Article in The American journal of tropical medicine and hygiene, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Diagnostic accuracy of non-culture methods for the diagnosis of melioidosis: a systematic review and meta-analysis.BMC infectious diseases · 2025Pooled it
- Outcomes of patients with suspected or confirmed melioidosis: A prospective multinational, multicenter, observational study.PLoS neglected tropical diseases · 2026Observational
- Melioidosis molecular diagnostics: An update.Virulence · 2025Review
- Laboratory diagnosis of melioidosis.PLoS neglected tropical diseases · 2025Review
- Identification ofMicrobiology spectrum · 2024Article
- Burkholderia pseudomallei and melioidosis.Nature reviews. Microbiology · 2024Review
- Development of a Novel Internally Controlled HrpB1 Gene-Based Real-Time qPCR Assay for Detection of Burkholderia pseudomallei.Molecular diagnosis & therapy · 2024Article
- Use of RecombinantPathogens (Basel, Switzerland) · 2021Article
- Human Melioidosis.Clinical microbiology reviews · 2020Review
- Sensitivity and specificity of a lateral flow immunoassay (LFI) in serum samples for diagnosis of melioidosis.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2018Observational
- Evaluation of a Rapid Diagnostic Test for Detection of Burkholderia pseudomallei in the Lao People's Democratic Republic.Journal of clinical microbiology · 2018Article
- Melioidosis.Nature reviews. Disease primers · 2018Review
- Rapid Filter-Based Detection and Culture of Burkholderia pseudomallei from Small Volumes of Urine.Journal of clinical microbiology · 2017Article
- Susceptibility of Clinical Isolates ofThe American journal of tropical medicine and hygiene · 2017Article
- Comparison of O-polysaccharide and hemolysin co-regulated protein as target antigens for serodiagnosis of melioidosis.PLoS neglected tropical diseases · 2017Article
- Investigation of host-pathogen interaction betweenCell & bioscience · 2017Article
- Burkholderia pseudomallei: Challenges for the Clinical Microbiology Laboratory.Journal of clinical microbiology · 2016Review
- Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2016Article
- Development of Rapid Enzyme-Linked Immunosorbent Assays for Detection of Antibodies to Burkholderia pseudomallei.Journal of clinical microbiology · 2016Article
- Melioidosis diagnostic workshop, 2013.Emerging infectious diseases · 2015Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The diagnosis of melioidosis depends on the culture of Burkholderia pseudomallei, which takes at least 48 hours. We used a polyclonal-FITC-based immunofluorescence microscopic assay (Pab-IFA) on clinical samples to provide a rapid presumptive diagnosis. This has limitations including photobleaching and batch-to-batch variability. This study evaluated an IFA based on a monoclonal antibody specific to B. pseudomallei (Mab-IFA) and Alexa Fluor 488. A diagnostic evaluation was performed on a prospective cohort of 951 consecutive patients with suspected melioidosis. A total of 1,407 samples were tested. Test accuracy was defined against culture as the gold standard, and was also compared against Pab-IFA. A total of 88 samples from 64 patients were culture positive for B. pseudomallei. The diagnostic sensitivity and specificity of the Mab-IFA was comparable to the Pab-IFA (48.4% versus 45.3% for sensitivity, and 99.8% versus 98.8% for specificity). We have incorporated the Mab-IFA into our routine practice.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.