ArticleThe Journal of antibiotics2014
Medium optimization of Streptomyces sp. 17944 for tirandamycin B production and isolation and structural elucidation of tirandamycins H, I and J.
Article in The Journal of antibiotics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
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- Engineering Polyketide Stereocenters with Ketoreductase Domain Exchanges.Journal of the American Chemical Society · 2025Article
- Machine learning-led semi-automated medium optimization reveals salt as key for flaviolin production in Pseudomonas putida.Communications biology · 2025Article
- Genome sequences of threeMicrobiology resource announcements · 2025Article
- Metabolic engineering strategies for naringenin production enhancement in Streptomyces albidoflavus J1074.Microbial cell factories · 2023Article
- Balancing Trade-Offs Imposed by Growth Media and Mass Spectrometry for Bacterial Exometabolomics.Applied and environmental microbiology · 2022Article
- Desert Environments Facilitate Unique Evolution of Biosynthetic Potential inMolecules (Basel, Switzerland) · 2021Article
- Characterization of Actinomycetes Strains Isolated from the Intestinal Tract and Feces of the Larvae of the Longhorn BeetleMicroorganisms · 2020Article
- The Biological and Chemical Diversity of Tetramic Acid Compounds from Marine-Derived Microorganisms.Marine drugs · 2020Review
- Article
- Short-chain ketone production by engineered polyketide synthases in Streptomyces albus.Nature communications · 2018Article
- Biological Significance of Marine Actinobacteria of East Coast of Andhra Pradesh, India.Frontiers in microbiology · 2017Article
- Identification of an unexpected shunt pathway product provides new insights into tirandamycin biosynthesis.Tetrahedron letters · 2016Article
- Multi-Response Optimization of Granaticinic Acid Production by Endophytic Streptomyces thermoviolaceus NT1, Using Response Surface Methodology.Bioengineering (Basel, Switzerland) · 2016Article
- A New Analogue of Echinomycin and a New Cyclic Dipeptide from a Marine-Derived Streptomyces sp. LS298.Marine drugs · 2015Article
- Asparagine requirement in Plasmodium berghei as a target to prevent malaria transmission and liver infections.Nature communications · 2015Article
- Angucyclines and Angucyclinones from Streptomyces sp. CB01913 Featuring C-Ring Cleavage and Expansion.Journal of natural products · 2015Article
- Aminoacyl-tRNA synthetases as drug targets in eukaryotic parasites.International journal for parasitology. Drugs and drug resistance · 2014Review
- Formulation and Statistical Optimization of Culture Medium for Improved Production of Antimicrobial Compound by Streptomyces sp. JAJ06.International journal of microbiology · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
We have recently isolated tirandamycin (TAM) B from Streptomyces sp. 17944 as a Brugia malayi AsnRS (BmAsnRS) inhibitor that efficiently kills the adult B. malayi parasites and does not exhibit general cytotoxicity to human hepatic cells. We now report (i) the comparison of metabolite profiles of S. sp. 17944 in six different media, (ii) identification of a medium enabling the production of TAM B as essentially the sole metabolite, and with improved titer, and (iii) isolation and structural elucidation of three new TAM congeners. These findings shed new insights into the structure-activity relationship of TAM B as a BmAsnRS inhibitor, highlighting the δ-hydroxymethyl-α,β-epoxyketone moiety as the critical pharmacophore, and should greatly facilitate the production and isolation of sufficient quantities of TAM B for further mechanistic and preclinical studies to advance the candidacy of TAM B as an antifilarial drug lead. The current study also serves as an excellent reminder that traditional medium and fermentation optimization should continue to be very effective in improving metabolite flux and titer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.