ArticleReproductive biology and endocrinology : RB&E2013
Transcriptome profiling of mice testes following low dose irradiation.
Article in Reproductive biology and endocrinology : RB&E, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 20 citations in OpenAlex.
- The hydrogen storage nanomaterial MgHBiomaterials research · 2022Article
- Unexpected Interacting Effects of Physical (Radiation) and Chemical (Bisphenol A) Treatments on Male Reproductive Functions in Mice.International journal of molecular sciences · 2021Article
- A Testis-Specific Long Noncoding RNA,Frontiers in endocrinology · 2021Article
- Low-Dose Radiation Exposure withInternational journal of molecular sciences · 2020Article
- Wigwams: identifying gene modules co-regulated across multiple biological conditions.Bioinformatics (Oxford, England) · 2014Article
- mRNA and lncRNA Expression Profiling of Radiation-Induced Gastric Injury Reveals Potential Radiation-Responsive Transcription Factors.Dose-response : a publication of International Hormesis SocietyArticle
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8 authors at 3 institutions in 2 countries.
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Abstract
backgroundRadiotherapy is used routinely to treat testicular cancer. Testicular cells vary in radio-sensitivity and the aim of this study was to investigate cellular and molecular changes caused by low dose irradiation of mice testis and to identify transcripts from different cell types in the adult testis.
methodsTranscriptome profiling was performed on total RNA from testes sampled at various time points (n = 17) after 1 Gy of irradiation. Transcripts displaying large overall expression changes during the time series, but small expression changes between neighbouring time points were selected for further analysis. These transcripts were separated into clusters and their cellular origin was determined. Immunohistochemistry and in silico quantification was further used to study cellular changes post-irradiation (pi).
resultsWe identified a subset of transcripts (n = 988) where changes in expression pi can be explained by changes in cellularity. We separated the transcripts into five unique clusters that we associated with spermatogonia, spermatocytes, early spermatids, late spermatids and somatic cells, respectively. Transcripts in the somatic cell cluster showed large changes in expression pi, mainly caused by changes in cellularity. Further investigations revealed that the low dose irradiation seemed to cause Leydig cell hyperplasia, which contributed to the detected expression changes in the somatic cell cluster.
conclusionsThe five clusters represent gene expression in distinct cell types of the adult testis. We observed large expression changes in the somatic cell profile, which mainly could be attributed to changes in cellularity, but hyperplasia of Leydig cells may also play a role. We speculate that the possible hyperplasia may be caused by lower testosterone production and inadequate inhibin signalling due to missing germ cells.
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