ArticleStem cells (Dayton, Ohio)2013
Regulation of trachebronchial tissue-specific stem cell pool size.
Article in Stem cells (Dayton, Ohio), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Biobanked tracheal basal cells retain the capacity to differentiate.Laryngoscope investigative otolaryngology · 2022Article
- Biofilm aggregates and the host airway-microbial interface.Frontiers in cellular and infection microbiology · 2022Review
- Repeated injury promotes tracheobronchial tissue stem cell attrition.Stem cells translational medicine · 2021Article
- Lef-1 controls cell cycle progression in airway basal cells to regulate proliferation and differentiation.Stem cells (Dayton, Ohio) · 2021Article
- Airway epithelial stem cell chimerism in cystic fibrosis lung transplant recipients.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2021Article
- Electrospun scaffolds limit the regenerative potential of the airway epithelium.Laryngoscope investigative otolaryngology · 2019Article
- Cell Therapy for Cystic Fibrosis Lung Disease: Regenerative Basal Cell Amplification.Stem cells translational medicine · 2019Article
- Heterogeneity of Pulmonary Stem Cells.Advances in experimental medicine and biology · 2019Review
- Exhaustion of Airway Basal Progenitor Cells in Early and Established Chronic Obstructive Pulmonary Disease.American journal of respiratory and critical care medicine · 2018Article
- Opposing effects of in vitro differentiated macrophages sub-type on epithelial wound healing.PloS one · 2017Article
- Transplantation of Airway Epithelial Stem/Progenitor Cells: A Future for Cell-Based Therapy.American journal of respiratory cell and molecular biology · 2017Article
- What we can learn from a tadpole about ciliopathies and airway diseases: Using systems biology in Xenopus to study cilia and mucociliary epithelia.Genesis (New York, N.Y. : 2000) · 2017Review
- Wnt Signaling Regulates Airway Epithelial Stem Cells in Adult Murine Submucosal Glands.Stem cells (Dayton, Ohio) · 2016Article
- Airway Progenitor Clone Formation Is Enhanced by Y-27632-Dependent Changes in the Transcriptome.American journal of respiratory cell and molecular biology · 2016Article
- Near Equilibrium Calculus of Stem Cells in Application to the Airway Epithelium Lineage.PLoS computational biology · 2016Article
- Injury induces direct lineage segregation of functionally distinct airway basal stem/progenitor cell subpopulations.Cell stem cell · 2015Article
- Tracheal dysplasia precedes bronchial dysplasia in mouse model of N-nitroso trischloroethylurea induced squamous cell lung cancer.PloS one · 2015Article
- Epidermal growth factor receptor activity is necessary for mouse basal cell proliferation.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Progenitor cells in proximal airway epithelial development and regeneration.Journal of cellular biochemistry · 2014Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Tissue-specific stem cell (TSC) number is tightly regulated in normal individuals but can change following severe injury. We previously showed that tracheobronchial epithelial TSC number increased after severe naphthalene (NA) injury and then returned to normal. This study focused on the fate of the supernumerary TSC and the signals that regulate TSC pool size. We used the Keratin 5-rTA/Histone 2B:green fluorescent protein (GFP) model to purify basal cells that proliferated infrequently (GFP(bright) ) or frequently (GFP(dim) ) after NA injury. Both populations contained TSC but TSCs were 8.5-fold more abundant in the GFP(bright) population. Interestingly, both populations also contained a unipotential basal progenitor (UPB), a mitotic basal cell subtype whose daughters were terminally differentiated basal cells. The ratio of TSC to UPB was 5:1 in the GFP(bright) population and 1:5 in the GFP(dim) population. These data suggested that TSC proliferation in vivo promoted TSC-to-UPB differentiation. To evaluate this question, we cloned TSC from the GFP(bright) and GFP(dim) populations and passaged the clones seven times. We found that TSC number decreased and UPB number increased at each passage. Reciprocal changes in TSC and UPB frequency were more dramatic in the GFP(dim) lineage. Gene expression analysis showed that β-catenin and Notch pathway genes were differentially expressed in freshly isolated TSC derived from GFP(bright) and GFP(dim) populations. We conclude that (a) TSC and UPB are members of a single lineage; (b) TSC proliferation in vivo or in vitro promotes TSC-to-UPB differentiation; and (c) an interaction between the β-catenin and Notch pathways regulates the TSC-to-UPB differentiation process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.