ArticleThe Journal of cell biology2013
Cryo-electron tomography reveals a critical role of RIM1α in synaptic vesicle tethering.
Article in The Journal of cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed, 134 citations in OpenAlex.
- The missing link: Piccolino is essential for tethering synaptic vesicles to rod photoreceptor ribbons.The Journal of cell biology · 2026Article
- Resolving endogenous protein organization in cells with nanometer resolution.Nature communications · 2026Article
- Visualizing Postsynaptic Density in Excitatory Synapses with Electron Tomography.Advances in neurobiology · 2026Review
- Formation, Organization, and Maintenance of Synaptic Vesicle Pools: From Molecules to Structure and Dynamics.Advances in neurobiology · 2026Review
- Recent Structural Insights into the Molecular Architecture of Synapses.Advances in neurobiology · 2026Review
- Cryo-correlative light and electron tomography of dopaminergic axonal varicosities reveals non-synaptic modulation of cortico-striatal synapses.Nature communications · 2025Article
- Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons.Nature communications · 2025Article
- "Kiss-shrink-run" unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling.Science (New York, N.Y.) · 2025Article
- Number and relative abundance of synaptic vesicles in functionally distinct priming states determine synaptic strength and short-term plasticity.The Journal of physiology · 2025Article
- Independently engaging protein tethers of different length enhance synaptic vesicle trafficking to the plasma membrane.The Journal of physiology · 2025Article
- The synaptic vesicle cluster as a controller of pre- and postsynaptic structure and function.The Journal of physiology · 2025Review
- SynapseNet: Deep learning for automatic synapse reconstruction.Molecular biology of the cell · 2025Article
- RIM and MUNC13 membrane-binding domains are essential for neuropeptide secretion.The Journal of cell biology · 2025Article
- The postsynaptic density in excitatory synapses is composed of clustered, heterogeneous nanoblocks.The Journal of cell biology · 2025Article
- Nanoscale architecture of synaptic vesicles and scaffolding complexes revealed by cryo-electron tomography.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Nanoscaled RIM clustering at presynaptic active zones revealed by endogenous tagging.Life science alliance · 2023Article
- Munc13- and SNAP25-dependent molecular bridges play a key role in synaptic vesicle priming.Science advances · 2023Article
- Article
- The activation of mGluR4 rescues parallel fiber synaptic transmission and LTP, motor learning and social behavior in a mouse model of Fragile X Syndrome.Molecular autism · 2023Article
- Optogenetics and electron tomography for structure-function analysis of cochlear ribbon synapses.eLife · 2022Article
25 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synaptic vesicles are embedded in a complex filamentous network at the presynaptic terminal. Before fusion, vesicles are linked to the active zone (AZ) by short filaments (tethers). The identity of the molecules that form and regulate tethers remains unknown, but Rab3-interacting molecule (RIM) is a prominent candidate, given its central role in AZ organization. In this paper, we analyzed presynaptic architecture of RIM1α knockout (KO) mice by cryo-electron tomography. In stark contrast to previous work on dehydrated, chemically fixed samples, our data show significant alterations in vesicle distribution and AZ tethering that could provide a structural basis for the functional deficits of RIM1α KO synapses. Proteasome inhibition reversed these structural defects, suggesting a functional recovery confirmed by electrophysiological recordings. Altogether, our results not only point to the ubiquitin-proteasome system as an important regulator of presynaptic architecture and function but also show that the tethering machinery plays a critical role in exocytosis, converging into a structural model of synaptic vesicle priming by RIM1α.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.