ArticleAnalytical biochemistry2013
Improving the thermal, radial, and temporal accuracy of the analytical ultracentrifuge through external references.
Article in Analytical biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
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- Modulation of Biophysical Properties of Nucleocapsid Protein in the Mutant Spectrum of SARS-CoV-2.bioRxiv : the preprint server for biology · 2024Article
- Amyloid-β aggregates activate peripheral monocytes in mild cognitive impairment.Nature communications · 2024Article
- Assembly reactions of SARS-CoV-2 nucleocapsid protein with nucleic acid.bioRxiv : the preprint server for biology · 2023Article
- Best Practices for Aggregate Quantitation of Antibody Therapeutics by Sedimentation Velocity Analytical Ultracentrifugation.Journal of pharmaceutical sciences · 2022Review
- The dimerization interface of initiator RctB governs chaperone and enhancer dependence of Vibrio cholerae chromosome 2 replication.Nucleic acids research · 2022Article
- Molecular insight into 2-phosphoglycolate activation of the phosphatase activity of bisphosphoglycerate mutase.Acta crystallographica. Section D, Structural biology · 2022Article
- Characterization of DNA-protein complexes by nanoparticle tracking analysis and their association with systemic lupus erythematosus.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Analytical band centrifugation for the separation and quantification of empty and full AAV particles.Molecular therapy. Methods & clinical development · 2021Article
- Calibrating analytical ultracentrifuges.European biophysics journal : EBJ · 2021Article
- Resuspending samples in analytical ultracentrifugation.Analytical biochemistry · 2020Article
- Quantitative Analysis of Protein Self-Association by Sedimentation Velocity.Current protocols in protein science · 2020Article
- Hybrid Thermophilic/Mesophilic Enzymes Reveal a Role for Conformational Disorder in Regulation of Bacterial Enzyme I.Journal of molecular biology · 2020Article
- Bacillus anthracis Virulence Regulator AtxA Binds Specifically to theJournal of bacteriology · 2019Article
- Nucleic acid-induced dimerization of HIV-1 Gag protein.The Journal of biological chemistry · 2019Article
- Cryo-EM structure of substrate-freeCurrent research in structural biology · 2019Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Sedimentation velocity (SV) is a method based on first principles that provides a precise hydrodynamic characterization of macromolecules in solution. Due to recent improvements in data analysis, the accuracy of experimental SV data emerges as a limiting factor in its interpretation. Our goal was to unravel the sources of experimental error and develop improved calibration procedures. We implemented the use of a Thermochron iButton temperature logger to directly measure the temperature of a spinning rotor and detected deviations that can translate into an error of as much as 10% in the sedimentation coefficient. We further designed a precision mask with equidistant markers to correct for instrumental errors in the radial calibration that were observed to span a range of 8.6%. The need for an independent time calibration emerged with use of the current data acquisition software (Zhao et al., Anal. Biochem., 437 (2013) 104-108), and we now show that smaller but significant time errors of up to 2% also occur with earlier versions. After application of these calibration corrections, the sedimentation coefficients obtained from 11 instruments displayed a significantly reduced standard deviation of approximately 0.7%. This study demonstrates the need for external calibration procedures and regular control experiments with a sedimentation coefficient standard.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.