ArticleJournal of visualized experiments : JoVE2013
Untargeted metabolomics from biological sources using ultraperformance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS).
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- Human follicular fluid shows diverse metabolic profiles at different follicle developmental stages.Reproductive biology and endocrinology : RB&E · 2020Trial
- Marine Antimicrobial Peptides: From Ocean Biodiversity to Genome Mining, Multi-Omics Discovery, and Biotechnological Innovation in the Battle Against Antimicrobial Resistance.Probiotics and antimicrobial proteins · 2026Review
- Untargeted mass spectrometry to investigate ocean acidification inThe Analyst · 2026Article
- Dengue virus infection in children: Serum lipidomics profiling for biomarker discovery.PLoS neglected tropical diseases · 2025Article
- Altered lipid homeostasis is associated with cerebellar neurodegeneration in SNX14 deficiency.JCI insight · 2024Article
- Biomaterials and Bioactive Natural Products from Marine Invertebrates: From Basic Research to Innovative Applications.Marine drugs · 2022Review
- Metabonomics reveals that entomopathogenic nematodes mediate tryptophan metabolites that kill host insects.Frontiers in microbiology · 2022Article
- Primary saturation of α, β-unsaturated carbonyl containing fatty acids does not abolish electrophilicity.Chemico-biological interactions · 2021Article
- A Protocol for Untargeted Metabolomic Analysis: From Sample Preparation to Data Processing.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Lipid Synthesis Is Required to Resolve Endoplasmic Reticulum Stress and Limit Fibrotic Responses in the Lung.American journal of respiratory cell and molecular biology · 2018Article
- Metabolomic analysis of mouse prefrontal cortex reveals upregulated analytes during wakefulness compared to sleep.Scientific reports · 2018Article
- Hyper response to ovarian stimulation affects the follicular fluid metabolomic profile of women undergoing IVF similarly to polycystic ovary syndrome.Metabolomics : Official journal of the Metabolomic Society · 2018Article
- Comparison of statistical methods for detection of serum lipid biomarkers for mesothelioma and asbestos exposure.Biomarkers in medicine · 2017Article
- Differences in testosterone and its precursors by sex of the offspring in meconium.The Journal of steroid biochemistry and molecular biology · 2017Article
- Cytotoxic 1-deoxysphingolipids are metabolized by a cytochrome P450-dependent pathway.Journal of lipid research · 2017Article
- Effects of MeJA on Arabidopsis metabolome under endogenous JA deficiency.Scientific reports · 2016Article
- The use of mass spectrometry for analysing metabolite biomarkers in epidemiology: methodological and statistical considerations for application to large numbers of biological samples.European journal of epidemiology · 2016Review
- Company profile: BluePen Biomarkers LLC – integrated biomarker solutions.Future science OA · 2016Article
- Evidence for Intramyocardial Disruption of Lipid Metabolism and Increased Myocardial Ketone Utilization in Advanced Human Heart Failure.Circulation · 2016Article
- Production of stable isotope-labeled acyl-coenzyme A thioesters by yeast stable isotope labeling by essential nutrients in cell culture.Analytical biochemistry · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Here we present a workflow to analyze the metabolic profiles for biological samples of interest including; cells, serum, or tissue. The sample is first separated into polar and non-polar fractions by a liquid-liquid phase extraction, and partially purified to facilitate downstream analysis. Both aqueous (polar metabolites) and organic (non-polar metabolites) phases of the initial extraction are processed to survey a broad range of metabolites. Metabolites are separated by different liquid chromatography methods based upon their partition properties. In this method, we present microflow ultra-performance (UP)LC methods, but the protocol is scalable to higher flows and lower pressures. Introduction into the mass spectrometer can be through either general or compound optimized source conditions. Detection of a broad range of ions is carried out in full scan mode in both positive and negative mode over a broad m/z range using high resolution on a recently calibrated instrument. Label-free differential analysis is carried out on bioinformatics platforms. Applications of this approach include metabolic pathway screening, biomarker discovery, and drug development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.