ArticleChemistry Central journal2013
Mechanistic study of a diazo dye degradation by Soybean Peroxidase.
Article in Chemistry Central journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 70 citations in OpenAlex.
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- Inactivation kinetics of horseradish peroxidase (HRP) by hydrogen peroxide.Scientific reports · 2023Article
- Citrus limon peroxidase-assisted biocatalytic approach for biodegradation of reactive 1847 colfax blue P3R and 621 colfax blue R dyes.Bioprocess and biosystems engineering · 2023Article
- Biocatalytic potential of Brassica oleracea L. var. botrytis leaves peroxidase for efficient degradation of textile dyes in aqueous medium.Bioprocess and biosystems engineering · 2023Article
- Environmental and molecular approach to dye industry waste degradation by the ascomycete fungus Nectriella pironii.Scientific reports · 2021Article
- Recent Achievements in Dyes Removal Focused on Advanced Oxidation Processes Integrated with Biological Methods.Molecules (Basel, Switzerland) · 2021Review
- Soybean Peroxidase Catalyzed Decoloration of Acid Azo Dyes.Journal of health & pollution · 2020Article
- Facile synthesis of laccase mimic Cu/HRSC advances · 2019Article
- Horseradish peroxidase-mediated decolourization of Orange II: modelling hydrogen peroxide utilization efficiency at different pH values.Environmental science and pollution research international · 2018Article
- Comparative Degradation of a Thiazole Pollutant by an Advanced Oxidation Process and an Enzymatic Approach.Biomolecules · 2017Article
- Differential Degradation and Detoxification of an Aromatic Pollutant by Two Different Peroxidases.Biomolecules · 2017Article
- Degradation of orange dyes and carbamazepine by soybean peroxidase immobilized on silica monoliths and titanium dioxide.Environmental science and pollution research international · 2016Article
- Immobilized Lignin Peroxidase-Like Metalloporphyrins as Reusable Catalysts in Oxidative Bleaching of Industrial Dyes.Molecules (Basel, Switzerland) · 2016Review
- Soybean peroxidase-mediated degradation of an azo dye- a detailed mechanistic study.BMC biochemistry · 2013Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEnzyme based remediation of wastewater is emerging as a novel, efficient and environmentally-friendlier approach. However, studies showing detailed mechanisms of enzyme mediated degradation of organic pollutants are not widely published.
resultsThe present report describes a detailed study on the use of Soybean Peroxidase to efficiently degrade Trypan Blue, a diazo dye. In addition to examining various parameters that can affect the dye degradation ability of the enzyme, such as enzyme and H2O2 concentration, reaction pH and temperature, we carried out a detailed mechanistic study of Trypan Blue degradation. HPLC-DAD and LC-MS/MS studies were carried out to confirm dye degradation and analyze the intermediate metabolites and develop a detailed mechanistic dye degradation pathway.
conclusionWe report that Soybean peroxidase causes Trypan Blue degradation via symmetrical azo bond cleavage and subsequent radical-initiated ring opening of the metabolites. Interestingly, our results also show that no high molecular weight polymers were produced during the peroxidase-H2O2 mediated degradation of the phenolic Trypan Blue.
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