ArticleBMB reports2013
Morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditions.
Article in BMB reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Evaluation of a human 3D multi-spheroid model derived from SH-SY5Y cells for cytotoxicity testing.Scientific reports · 2026Article
- Magnetic Levitational Assembly of Differentiated SH-SY5Y Cells for Aβ-Induced 3D Alzheimer's Disease Modeling and Curcumin Screening.Macromolecular bioscience · 2025Article
- Organic charge-modulated transistor for electrophysiological measurements of human-derived neurospheroids.Frontiers in bioengineering and biotechnology · 2025Article
- Article
- An Optical and Chemiluminescence Assay for Assessing the Cytotoxicity ofBioengineering (Basel, Switzerland) · 2022Article
- Balamuthia mandrillaris trophozoites ingest human neuronal cells via a trogocytosis-independent mechanism.Parasites & vectors · 2022Article
- Electrophysiology Read-Out Tools for Brain-on-Chip Biotechnology.Micromachines · 2021Review
- Spheroid Culture of Mammalian Olfactory Receptor Neurons: Potential Applications for a Bioelectronic Nose.Experimental neurobiology · 2018Article
- CDDO and ATRA Instigate Differentiation of IMR32 Human Neuroblastoma Cells.Frontiers in molecular neuroscience · 2017Article
- Ginsenoside-Rp1-induced apolipoprotein A-1 expression in the LoVo human colon cancer cell line.Journal of ginseng research · 2014Article
- 2-Cyclopropylimino-3-methyl-1,3-thiazoline hydrochloride protects against beta-amyloid-induced activation of the apoptotic cascade in cultured cortical neurons.Cellular and molecular neurobiology · 2014Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we aimed to compare the morphogenetic and neuronal characteristics between monolayer cells and spheroids. For this purpose, we established spheroid formation by growing SH-SY5Y cells on the hydrophobic surfaces of thermally-collapsed elastin-like polypeptide. After 4 days of culture, the relative proliferation of the cells within spheroids was approximately 92% of the values for monolayer cultures. As measured by quantitative assays for mRNA and protein expressions, the production of synaptophysin and neuronspecific enolase (NSE) as well as the contents of cell adhesion molecules (CAMs) and extracellular matrix (ECM) proteins are much higher in spheroids than in monolayer cells. Under the all-trans-retinoic acid (RA)-induced differentiation condition, spheroids extended neurites and further up-regulated the expression of synaptophysin, NSE, CAMs, and ECM proteins. Our data indicate that RA-differentiated SH-SY5Y neurospheroids are functionally matured neuronal architectures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.