ArticleACS nano2013
Selecting improved peptidyl motifs for cytosolic delivery of disparate protein and nanoparticle materials.
Article in ACS nano, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 131 citations in OpenAlex.
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- Combining nitric oxide and calcium sensing for the detection of endothelial dysfunction.Communications chemistry · 2023Review
- Self assembling nanoparticle enzyme clusters provide access to substrate channeling in multienzymatic cascades.Nature communications · 2023Article
- Determining the Cytosolic Stability of Small DNA NanostructuresNano letters · 2022Article
- Implementing Multi-Enzyme Biocatalytic Systems Using Nanoparticle Scaffolds.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Mitochondrial Targeting Probes, Drug Conjugates, and Gene Therapeutics.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Cytosolic delivery of membrane-penetrating QDs into T cell lymphocytes: implications in immunotherapy and drug delivery.Nanoscale · 2021Article
- Article
- Preparation and Characterization of Quantum Dot-Peptide Conjugates Based on Polyhistidine Tags.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Endothelial Cell Targeting by cRGD-Functionalized Polymeric Nanoparticles under Static and Flow Conditions.Nanomaterials (Basel, Switzerland) · 2020Article
- Bioluminescence-Based Energy Transfer Using Semiconductor Quantum Dots as Acceptors.Sensors (Basel, Switzerland) · 2020Review
- Nanoscale systems for local drug delivery.Nano today · 2019Article
- Selective Cytotoxicity to HER2 Positive Breast Cancer Cells by Saporin-Loaded Nanobody-Targeted Polymeric Nanoparticles in Combination with Photochemical Internalization.Molecular pharmaceutics · 2019Article
- Cholesterol Functionalization of Gold Nanoparticles Enhances Photoactivation of Neural Activity.ACS chemical neuroscience · 2019Article
- Enhanced Uptake of Luminescent Quantum Dots by Live Cells Mediated by a Membrane-Active Peptide.ACS omega · 2018Article
- Evaluating the potential of using quantum dots for monitoring electrical signals in neurons.Nature nanotechnology · 2018Review
- A method for estimating intracellular ion concentration using optical nanosensors and ratiometric imaging.Scientific reports · 2017Article
- Quantum Dot-Peptide-Fullerene Bioconjugates for Visualization of in Vitro and in Vivo Cellular Membrane Potential.ACS nano · 2017Article
Corrections and comments
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Authors and funding
20 authors at 5 institutions in 2 countries.
Funding
Abstract
Cell penetrating peptides facilitate efficient intracellular uptake of diverse materials ranging from small contrast agents to larger proteins and nanoparticles. However, a significant impediment remains in the subsequent compartmentalization/endosomal sequestration of most of these cargoes. Previous functional screening suggested that a modular peptide originally designed to deliver palmitoyl-protein thioesterase inhibitors to neurons could mediate endosomal escape in cultured cells. Here, we detail properties relevant to this peptide's ability to mediate cytosolic delivery of quantum dots (QDs) to a wide range of cell-types, brain tissue culture and a developing chick embryo in a remarkably nontoxic manner. The peptide further facilitated efficient endosomal escape of large proteins, dendrimers and other nanoparticle materials. We undertook an iterative structure-activity relationship analysis of the peptide by discretely modifying key components including length, charge, fatty acid content and their order using a comparative, semiquantitative assay. This approach allowed us to define the key motifs required for endosomal escape, to select more efficient escape sequences, along with unexpectedly identifying a sequence modified by one methylene group that specifically targeted QDs to cellular membranes. We interpret our results within a model of peptide function and highlight implications for in vivo labeling and nanoparticle-mediated drug delivery by using different peptides to co-deliver cargoes to cells and engage in multifunctional labeling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.