ArticleBioMed research international2013
Development of loop-mediated isothermal amplification for detection of Leifsonia xyli subsp. xyli in sugarcane.
Article in BioMed research international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 38 citations in OpenAlex.
- Innovative Colorimetric Neutral Red-Based Loop-Mediated Isothermal Amplification (NR-LAMP) Assay: Transforming Rapid and Affordable Feline Leukemia Virus Detection.International journal of molecular sciences · 2025Article
- Article
- Evaluation of molecular inhibitors of loop-mediated isothermal amplification (LAMP).Scientific reports · 2024Article
- StratoLAMP: Label-free, multiplex digital loop-mediated isothermal amplification based on visual stratification of precipitate.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Mayaro virus detection by integrating sample preparation with isothermal amplification in portable devices.Analytical and bioanalytical chemistry · 2023Article
- Rapid Detection of Hepatitis A Virus in Foods Using a Bioluminescent Assay in Real-Time (BART) and Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Technology.Food and environmental virology · 2023Article
- Changing the diagnostic paradigm for sugarcane: development of a mill-based diagnostic for ratoon stunting disease in crude cane juice.Frontiers in plant science · 2023Article
- Molecular characterization of strawberry vein banding virus from China and the development of loop‑mediated isothermal amplification assays for their detection.Scientific reports · 2022Article
- Development of a loop-mediated isothermal amplification (LAMP) assay for the detection of Anaplasma marginale.Experimental & applied acarology · 2019Article
- A Comparative Study of Three Detection Techniques forBioMed research international · 2018Article
- Development and application of a rapid and visual loop-mediated isothermal amplification for the detection of Sporisorium scitamineum in sugarcane.Scientific reports · 2016Article
- Detection of Puccinia kuehnii Causing Sugarcane Orange Rust with a Loop-Mediated Isothermal Amplification-Based Assay.Molecular biotechnology · 2016Article
- Rapid and Quantitative Detection ofBioMed research international · 2016Article
- Loop-mediated isothermal amplification (LAMP) based detection of Colletotrichum falcatum causing red rot in sugarcane.Molecular biology reports · 2015Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Ratoon stunt, caused by the xylem-limited coryneform bacterium Leifsonia xyli subsp. xyli (Lxx), is a deep bacteriosis and prevalent in most of sugarcane-producing countries. Based on loop-mediated isothermal amplification (LAMP), we developed a method for detecting Lxx. The major advantages of the LAMP method are visual judgment by color and time saving with only 60 min for identification of Lxx and without the need for costly PCR apparatus and gel scanner. In the present study, positive and negative samples detected by the LAMP method were clearly distinguishable. When total DNA extracted from internode juice was used as the template, the sensitivity of LAMP was 10 times higher than that of the conventional PCR detection. The LAMP assay is a highly specific, rapid, and sensitive method for the diagnosis of ratoon stunt caused by Lxx in sugarcane. This is the first report of LAMP-based assay for the detection of Lxx in sugarcane.
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