ArticlePlant physiology2013
Comprehensive investigation of microRNAs enhanced by analysis of sequence variants, expression patterns, ARGONAUTE loading, and target cleavage.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 69 citations in OpenAlex.
- Exosome-like nanoparticles derived from fruits, vegetables, and herbs: innovative strategies of therapeutic and drug delivery.Theranostics · 2024Pooled it
- HASTY-dependent miRNA cell-to-cell movement is required for systemic pathogen resistance in Arabidopsis.Plant communications · 2026Article
- Transcriptome-wide analysis of Arabidopsis DICER-LIKE1 RNA substrates.Nucleic acids research · 2026Article
- Somatic DNA demethylation generates tissue-specific methylation states and impacts flowering time.The Plant cell · 2022Article
- Regulatory non-coding RNAs: a new frontier in regulation of plant biology.Functional & integrative genomics · 2021Review
- Barley Seeds miRNome Stability during Long-Term Storage and Aging.International journal of molecular sciences · 2021Article
- Novel approaches on identification of conserved miRNAs for broad-spectrum Potyvirus control measures.Molecular biology reports · 2021Article
- MicroRNA Profiling During Mulberry (Frontiers in plant science · 2021Article
- Genome-wide analysis of brassinosteroid responsive small RNAs in Arabidopsis thaliana.Genes & genomics · 2020Article
- Investigation into the miRNA/5' isomiRNAs function and drought-mediated miRNA processing in rice.Functional & integrative genomics · 2020Article
- Diff isomiRs: Large-scale detection of differential isomiRs for understanding non-coding regulated stress omics in plants.Scientific reports · 2019Article
- Article
- MicroR408 regulates defense response upon wounding in sweet potato.Journal of experimental botany · 2019Article
- Plant 24-nt reproductive phasiRNAs from intramolecular duplex mRNAs in diverse monocots.Genome research · 2018Article
- MiRNA-seq-based profiles of miRNAs in mulberry phloem sap provide insight into the pathogenic mechanisms of mulberry yellow dwarf disease.Scientific reports · 2018Article
- Bioinformatics resources for deciphering the biogenesis and action pathways of plant small RNAs.Rice (New York, N.Y.) · 2017Review
- Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22.Scientific reports · 2017Article
- Loss of function of Arabidopsis microRNA-machinery genes impairs fertility, and has effects on homologous recombination and meiotic chromatin dynamics.Scientific reports · 2017Article
- Mutations inThe Plant cell · 2017Article
- Characterization and Function of MicroRNAFrontiers in plant science · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
MicroRNAs (miRNAs) are a class of small RNAs that typically function by guiding the cleavage of target messenger RNAs. They have been shown to play major roles in a variety of plant processes, including development, and responses to pathogens and environmental stresses. To identify new miRNAs and regulation in Arabidopsis (Arabidopsis thaliana), 27 small RNA libraries were constructed and sequenced from various tissues, stresses, and small RNA biogenesis mutants, resulting in 95 million genome-matched sequences. The use of rdr2 to enrich the miRNA population greatly enhanced this analysis and led to the discovery of new miRNAs arising from both known and new precursors, increasing the total number of Arabidopsis miRNAs by about 10%. Parallel Analysis of RNA Ends data provide evidence that the majority guide target cleavage. Many libraries represented novel stress/tissue conditions, such as submergence-stressed flowers, which enabled the identification of new stress regulation of both miRNAs and their targets, all of which were validated in wild-type plants. By combining small RNA expression analysis with ARGONAUTE immunoprecipitation data and global target cleavage data from Parallel Analysis of RNA Ends, a much more complete picture of Arabidopsis miRNAs was obtained. In particular, the discovery of ARGONAUTE loading and target cleavage biases gave important insights into tissue-specific expression patterns, pathogen responses, and the role of sequence variation among closely related miRNA family members that would not be evident without this combinatorial approach.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.