ArticlePlant physiology2013
A redox 2-Cys mechanism regulates the catalytic activity of divergent cyclophilins.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Structural and Kinetic Variability of Pathogen Cyclophilins: Functional Diversification and Therapeutic Potential.ACS infectious diseases · 2026Review
- Characterization and regulation of salt upregulated cyclophilin from a halotolerant strain of Penicillium oxalicum.Scientific reports · 2023Article
- Structural Basis for Cyclosporin Isoform-Specific Inhibition of Cyclophilins fromACS infectious diseases · 2023Article
- Cyclophilin anaCyp40 regulates photosystem assembly and phycobilisome association in a cyanobacterium.Nature communications · 2022Article
- Plant Cyclophilins: Multifaceted Proteins With Versatile Roles.Frontiers in plant science · 2020Review
- Genome-Wide Analysis of Cyclophilin Proteins in 21 Oomycetes.Pathogens (Basel, Switzerland) · 2019Article
- Ole e 15 and its human counterpart -PPIA- chimeras reveal an heterogeneous IgE response in olive pollen allergic patients.Scientific reports · 2019Article
- Human glutathione-S-transferase pi potentiates the cysteine-protease activity of the Der p 1 allergen from house dust mite through a cysteine redox mechanism.Redox biology · 2019Article
- Enzyme activity and structural features of three single-domain phloem cyclophilins from Brassica napus.Scientific reports · 2019Article
- Proteome-Wide Analysis of Cysteine Reactivity during Effector-Triggered Immunity.Plant physiology · 2019Article
- Whole genome SNP-associated signatures of local adaptation in honeybees of the Iberian Peninsula.Scientific reports · 2018Article
- Molecular cloning, expression, purification and functional characterization of an antifungal cyclophilin protein fromBiomedical reports · 2017Article
- Molecular characterization of XopAG effector AvrGf2 from Xanthomonas fuscans ssp. aurantifolii in grapefruit.Molecular plant pathology · 2017Article
- OsPEX11, a Peroxisomal Biogenesis Factor 11, Contributes to Salt Stress Tolerance inFrontiers in plant science · 2016Article
- Systemic Induction of NO-, Redox-, and cGMP Signaling in the Pumpkin Extrafascicular Phloem upon Local Leaf Wounding.Frontiers in plant science · 2016Article
- Article
- Identification of putative TAL effector targets of the citrus canker pathogens shows functional convergence underlying disease development and defense response.BMC genomics · 2014Article
- Citrus MAF1, a repressor of RNA polymerase III, binds the Xanthomonas citri canker elicitor PthA4 and suppresses citrus canker development.Plant physiology · 2013Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
The citrus (Citrus sinensis) cyclophilin CsCyp is a target of the Xanthomonas citri transcription activator-like effector PthA, required to elicit cankers on citrus. CsCyp binds the citrus thioredoxin CsTdx and the carboxyl-terminal domain of RNA polymerase II and is a divergent cyclophilin that carries the additional loop KSGKPLH, invariable cysteine (Cys) residues Cys-40 and Cys-168, and the conserved glutamate (Glu) Glu-83. Despite the suggested roles in ATP and metal binding, the functions of these unique structural elements remain unknown. Here, we show that the conserved Cys residues form a disulfide bond that inactivates the enzyme, whereas Glu-83, which belongs to the catalytic loop and is also critical for enzyme activity, is anchored to the divergent loop to maintain the active site open. In addition, we demonstrate that Cys-40 and Cys-168 are required for the interaction with CsTdx and that CsCyp binds the citrus carboxyl-terminal domain of RNA polymerase II YSPSAP repeat. Our data support a model where formation of the Cys-40-Cys-168 disulfide bond induces a conformational change that disrupts the interaction of the divergent and catalytic loops, via Glu-83, causing the active site to close. This suggests a new type of allosteric regulation in divergent cyclophilins, involving disulfide bond formation and a loop-displacement mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.