Evidence map›Paper›PMID 23709638›Full record

ArticleGenetics2013

Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease.

Scott J Gratz, Alexander M Cummings, Jennifer N Nguyen, Danielle C Hamm, Laura K Donohue, Melissa M Harrison, Jill Wildonger, Kate M O'Connor-Giles

Abstract read
In one paragraph

Article in Genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 604 papers.

0numbers the graph read from it
0cells of the map it votes in
604citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

604 citing papers in PubMed.

  1. Article
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  12. Single nucleotide variants inBiochemistry and biophysics reports · 2026
    Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Cloning-Free Genome Editing by CRISPR/T7RNAP/Cas9 in Trypanosoma cruzi.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  18. Article
  19. The use ofFrontiers in cell and developmental biology · 2026
    Review
  20. Review

544 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Scott J GratzGenetics Training Program, University of Wisconsin, Madison, Madison, WI 53706, USA.
Alexander M Cummings
Jennifer N Nguyen
Danielle C Hamm
Laura K Donohue
Melissa M Harrison
Jill Wildonger
Kate M O'Connor-Giles

Funding

PREDOCTORAL TRAINING PROGRAM IN GENETICST32GM007133 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI PERNA, NICOLE T · 1985 to 2023
$16.5M
Molecular mechanisms of synapse assembly and functionR01NS078179 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI O'CONNOR-GILES, KATHALEEN M · 2013 to 2023
$3.6M
Regulation of synaptic growth and plasticity in DrosophilaR00NS060985 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI O'CONNOR-GILES, KATHALEEN M · 2009 to 2011
$747k
ROLE OF MICROTUBULE-BASED TRANSPORT IN NEURONAL POLARITYR00NS072252 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI WILDONGER, JILL C · 2012 to 2014
$729k
NIGMS NIH HHS T32 GM007133NINDS NIH HHS R00 NS060985NINDS NIH HHS R00 NS072252NINDS NIH HHS R01 NS078179
6 · The paper itself

Abstract

We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.

Indexed as

CRISPR-Cas SystemsGenome, InsectAnimalsClustered Regularly Interspaced Short Palindromic RepeatsDrosophilaDrosophila ProteinsEndodeoxyribonucleasesGene TargetingGenetic EngineeringGerm-Line MutationHomologous RecombinationDrosophila ProteinsEndodeoxyribonucleasesy protein, DrosophilaCas9CRISPR RNADrosophilagenome engineeringhomologous recombination

Identifiers

PMID23709638
PMCPMC3730909

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.