ArticleGenome biology and evolution2013
Deep sequencing of mixed total DNA without barcodes allows efficient assembly of highly plastic ascidian mitochondrial genomes.
Article in Genome biology and evolution, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed.
- Site-specific variation and non-indigenous species detection in Arabian Gulf biofouling communities using DNA metabarcoding and photographic surveys.Scientific reports · 2026Article
- Phylogenetic Relationship and Characterization of the Complete Mitochondrial Genome of the Cuckoo SpeciesInternational journal of molecular sciences · 2025Article
- Morphological Characteristics, Mitochondrial Genome, and Evolutionary Insights Into a New Sea Squirt From the Beibu Gulf.Ecology and evolution · 2025Article
- Evolutionary Insights from the Mitochondrial Genome of Oikopleura dioica: Sequencing Challenges, RNA Editing, Gene Transfers to the Nucleus, and tRNA Loss.Genome biology and evolution · 2024Article
- Evolution of myxozoan mitochondrial genomes: insights from myxobolids.BMC genomics · 2024Article
- Complete sequence and comparative analysis of the mitochondrial genome of the rare and endangeredFrontiers in genetics · 2022Article
- The complete mitochondrial genome of the forest crested lizard,Mitochondrial DNA. Part B, Resources · 2022Article
- An elongated COI fragment to discriminate botryllid species and as an improved ascidian DNA barcode.Scientific reports · 2021Article
- Evolution of Allorecognition in the Tunicata.Biology · 2020Review
- Inferring Tunicate Relationships and the Evolution of the Tunicate Hox Cluster with the Genome of Corella inflata.Genome biology and evolution · 2020Article
- Mitochondrial genomes of stick insects (Phasmatodea) and phylogenetic considerations.PloS one · 2020Article
- Recent advances in understanding mitochondrial genome diversity.F1000Research · 2020Review
- Complete mitochondrial genome sequence of the Himalayan Griffon,Ecology and evolution · 2019Article
- A new mitochondrial gene order in the banded cusk-eelMitochondrial DNA. Part B, Resources · 2019Article
- The first complete mitochondrial genome sequence ofEcology and evolution · 2018Article
- Extensive mitochondrial gene rearrangements in Ctenophora: insights from benthic Platyctenida.BMC evolutionary biology · 2018Article
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- De novo draft assembly of the Botrylloides leachii genome provides further insight into tunicate evolution.Scientific reports · 2018Article
- Deep-level phylogeny of Cicadomorpha inferred from mitochondrial genomes sequenced by NGS.Scientific reports · 2017Article
- Article
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10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Ascidians or sea squirts form a diverse group within chordates, which includes a few thousand members of marine sessile filter-feeding animals. Their mitochondrial genomes are characterized by particularly high evolutionary rates and rampant gene rearrangements. This extreme variability complicates standard polymerase chain reaction (PCR) based techniques for molecular characterization studies, and consequently only a few complete Ascidian mitochondrial genome sequences are available. Using the standard PCR and Sanger sequencing approach, we produced the mitochondrial genome of Ascidiella aspersa only after a great effort. In contrast, we produced five additional mitogenomes (Botrylloides aff. leachii, Halocynthia spinosa, Polycarpa mytiligera, Pyura gangelion, and Rhodosoma turcicum) with a novel strategy, consisting in sequencing the pooled total DNA samples of these five species using one Illumina HiSeq 2000 flow cell lane. Each mitogenome was efficiently assembled in a single contig using de novo transcriptome assembly, as de novo genome assembly generally performed poorly for this task. Each of the new six mitogenomes presents a different and novel gene order, showing that no syntenic block has been conserved at the ordinal level (in Stolidobranchia and in Phlebobranchia). Phylogenetic analyses support the paraphyly of both Ascidiacea and Phlebobranchia, with Thaliacea nested inside Phlebobranchia, although the deepest nodes of the Phlebobranchia-Thaliacea clade are not well resolved. The strategy described here thus provides a cost-effective approach to obtain complete mitogenomes characterized by a highly plastic gene order and a fast nucleotide/amino acid substitution rate.
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