ArticleApplied and environmental microbiology2013
Precipitation of iron on the surface of Leptospira interrogans is associated with mutation of the stress response metalloprotease HtpX.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 12 citations in OpenAlex.
- Article
- Membrane Vesicle Production as a Bacterial Defense Against Stress.Frontiers in microbiology · 2020Review
- Characterizing interactions of Leptospira interrogans with proximal renal tubule epithelial cells.BMC microbiology · 2018Article
- Versatile effects of bacterium-released membrane vesicles on mammalian cells and infectious/inflammatory diseases.Acta pharmacologica Sinica · 2018Review
- Micronutrients and Leptospirosis: A Review of the Current Evidence.PLoS neglected tropical diseases · 2016Review
- Outer-membrane vesicles from Gram-negative bacteria: biogenesis and functions.Nature reviews. Microbiology · 2015Review
- Modulation of bacterial outer membrane vesicle production by envelope structure and content.BMC microbiology · 2014Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High concentrations of free metal ions in the environment can be detrimental to bacterial survival. However, bacteria utilize strategies, including the activation of stress response pathways and immobilizing chemical elements on their surface, to limit this toxicity. In this study, we characterized LA4131, the HtpX-like M48 metalloprotease from Leptospira interrogans, with a putative role in bacterial stress response and membrane homeostasis. Growth of the la4131 transposon mutant strain (L522) in 360 μM FeSO4 (10-fold the normal in vitro concentration) resulted in the production of an amorphous iron precipitate. Atomic force microscopy and transmission electron microscopy analysis of the strain demonstrated that precipitate production was associated with the generation and release of outer membrane vesicles (OMVs) from the leptospiral surface. Transcriptional studies indicated that inactivation of la4131 resulted in altered expression of a subset of metal toxicity and stress response genes. Combining these findings, this report describes OMV production in response to environmental stressors and associates OMV production with the in vitro activity of an HtpX-like metalloprotease.
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Registered trials
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