ArticleThe Journal of biological chemistry2013
ubiI, a new gene in Escherichia coli coenzyme Q biosynthesis, is involved in aerobic C5-hydroxylation.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 54 citations in OpenAlex.
- Global distribution of isoprenoid quinones across Bacteria.mSystems · 2026Article
- Heterologous plastoquinone production using a newly identified OThe FEBS journal · 2026Article
- FgUbiH Is Essential for Vegetative Development, Energy Metabolism, and Antioxidant Activity inMicroorganisms · 2024Article
- Understanding coenzyme Q.Physiological reviews · 2024Review
- An organic O donor for biological hydroxylation reactions.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- UbiN, a novel Rhodobacter capsulatus decarboxylative hydroxylase involved in aerobic ubiquinone biosynthesis.FEBS open bio · 2023Article
- Diversification of Ubiquinone Biosynthesis via Gene Duplications, Transfers, Losses, and Parallel Evolution.Molecular biology and evolution · 2023Article
- Role of themBio · 2023Article
- Long-Chain Molecules with Agro-Bioactivities and Their Applications.Molecules (Basel, Switzerland) · 2023Review
- Mining the Flavoproteome of Brucella ovis, the Brucellosis Causing Agent in Ovis aries.Microbiology spectrum · 2022Article
- The Biosynthetic Pathway of Ubiquinone Contributes to Pathogenicity of Francisella novicida.Journal of bacteriology · 2021Article
- A dedicated flavin-dependent monooxygenase catalyzes the hydroxylation of demethoxyubiquinone into ubiquinone (coenzyme Q) in Arabidopsis.The Journal of biological chemistry · 2021Article
- The EcoCyc Database in 2021.Frontiers in microbiology · 2021Article
- Coenzyme QFrontiers in bioengineering and biotechnology · 2021Article
- The OThe Journal of biological chemistry · 2020Article
- The Paradox of Coenzyme QNutrients · 2019Review
- Ubiquinone Biosynthesis over the Entire OmBio · 2019Article
- Coenzyme Q biosynthesis in the biopesticide Shenqinmycin-producing Pseudomonas aeruginosa strain M18.Journal of industrial microbiology & biotechnology · 2019Article
- A genome-wide screen inThe Journal of biological chemistry · 2017Article
- A Journey across Genomes Uncovers the Origin of Ubiquinone in Cyanobacteria.Genome biology and evolution · 2017Article
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Authors and funding
15 authors at 8 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coenzyme Q (ubiquinone or Q) is a redox-active lipid found in organisms ranging from bacteria to mammals in which it plays a crucial role in energy-generating processes. Q biosynthesis is a complex pathway that involves multiple proteins. In this work, we show that the uncharacterized conserved visC gene is involved in Q biosynthesis in Escherichia coli, and we have renamed it ubiI. Based on genetic and biochemical experiments, we establish that the UbiI protein functions in the C5-hydroxylation reaction. A strain deficient in ubiI has a low level of Q and accumulates a compound derived from the Q biosynthetic pathway, which we purified and characterized. We also demonstrate that UbiI is only implicated in aerobic Q biosynthesis and that an alternative enzyme catalyzes the C5-hydroxylation reaction in the absence of oxygen. We have solved the crystal structure of a truncated form of UbiI. This structure shares many features with the canonical FAD-dependent para-hydroxybenzoate hydroxylase and represents the first structural characterization of a monooxygenase involved in Q biosynthesis. Site-directed mutagenesis confirms that residues of the flavin binding pocket of UbiI are important for activity. With our identification of UbiI, the three monooxygenases necessary for aerobic Q biosynthesis in E. coli are known.
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