ArticleBiophysical journal2013
DNA ejection from an archaeal virus--a single-molecule approach.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 42 citations in OpenAlex.
- Two coinfecting archaeal viruses provide insights into virus-virus interactions.Communications biology · 2026Article
- Archaeal virus entry and egress.microLife · 2024Review
- 'SAXS-osmometer' method provides measurement of DNA pressure in viral capsids and delivers an empirical equation of state.Nucleic acids research · 2023Article
- Temperature-induced DNA density transition in phage λ capsid revealed with contrast-matching SANS.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- AT-specific DNA visualization revisits the directionality of bacteriophage λ DNA ejection.Nucleic acids research · 2023Article
- Review
- Physics of viral dynamics.Nature reviews. Physics · 2021Review
- Single-particle virology.Biophysical reviews · 2020Review
- Pressure-driven release of viral genome into a host nucleus is a mechanism leading to herpes infection.eLife · 2019Article
- Influence of Microscopic Interactions on the Flexible Mechanical Properties of Viral DNA.Biophysical journal · 2018Article
- Article
- Smectic viral capsids and the aneurysm instability.Journal of physics. Condensed matter : an Institute of Physics journal · 2018Article
- Structural studies ofProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Single-molecule measurements of viral ssRNA packaging.RNA (New York, N.Y.) · 2017Article
- Temperature and pH dependence of DNA ejection from archaeal lemon-shaped virus His1.European biophysics journal : EBJ · 2016Article
- Article
- Sulfolobus Spindle-Shaped Virus 1 Contains Glycosylated Capsid Proteins, a Cellular Chromatin Protein, and Host-Derived Lipids.Journal of virology · 2015Article
- Influence of Internal DNA Pressure on Stability and Infectivity of Phage λ.Journal of molecular biology · 2015Article
- Exploring the Balance between DNA Pressure and Capsid Stability in Herpesviruses and Phages.Journal of virology · 2015Article
- Archaeal extrachromosomal genetic elements.Microbiology and molecular biology reviews : MMBR · 2015Review
Corrections and comments
- Commented on by
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The translocation of genetic material from the viral capsid to the cell is an essential part of the viral infection process. Whether the energetics of this process is driven by the energy stored within the confined nucleic acid or cellular processes pull the genome into the cell has been the subject of discussion. However, in vitro studies of genome ejection have been limited to a few head-tailed bacteriophages with a double-stranded DNA genome. Here we describe a DNA release system that operates in an archaeal virus. This virus infects an archaeon Haloarcula hispanica that was isolated from a hypersaline environment. The DNA-ejection velocity of His1, determined by single-molecule experiments, is comparable to that of bacterial viruses. We found that the ejection process is modulated by the external osmotic pressure (polyethylene glycol (PEG)) and by increased ion (Mg(2+) and Na(+)) concentration. The observed ejection was unidirectional, randomly paused, and incomplete, which suggests that cellular processes are required to complete the DNA transfer.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.