ArticleBiophysical journal2013
Bilayer asymmetry influences integrin sequestering in raft-mimicking lipid mixtures.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- High spatial-resolved heat manipulating membrane heterogeneity alters cellular migration and signaling.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- ABCA1 transporter promotes the motility of human melanoma cells by modulating their plasma membrane organization.Biological research · 2023Article
- Galectin-3 Binding to αThe journal of physical chemistry. B · 2022Article
- Multimodal Investigation into the Interaction of Quinacrine with Microcavity-Supported Lipid Bilayers.Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Absence of Cardiolipin From the Outer Leaflet of a Mitochondrial Inner Membrane Mimic Restricts Opa1-Mediated Fusion.Frontiers in molecular biosciences · 2021Article
- Roles of Membrane Domains in Integrin-Mediated Cell Adhesion.International journal of molecular sciences · 2020Review
- Plasma membranes are asymmetric in lipid unsaturation, packing and protein shape.Nature chemical biology · 2020Article
- Localization of Double Bonds in Bacterial Glycerophospholipids Using 193 nm Ultraviolet Photodissociation in the Negative Mode.Analytical chemistry · 2020Article
- Gramicidin Increases Lipid Flip-Flop in Symmetric and Asymmetric Lipid Vesicles.Biophysical journal · 2019Article
- Preparation of asymmetric phospholipid vesicles for use as cell membrane models.Nature protocols · 2018Article
- Changes in Cholesterol Level Alter Integrin Sequestration in Raft-Mimicking Lipid Mixtures.Biophysical journal · 2018Article
- Structural Characterization of Phosphatidylcholines Using 193 nm Ultraviolet Photodissociation Mass Spectrometry.Analytical chemistry · 2017Article
- Topologically Diverse Human Membrane Proteins Partition to Liquid-Disordered Domains in Phase-Separated Lipid Vesicles.Biochemistry · 2016Article
- The extracellular matrix modulates H2O2 degradation and redox signaling in endothelial cells.Redox biology · 2015Article
- Ligand binding alters dimerization and sequestering of urokinase receptors in raft-mimicking lipid mixtures.Biophysical journal · 2014Article
- Lipid directed intrinsic membrane protein segregation.Journal of the American Chemical Society · 2013Article
- Model cell membranes: Techniques to form complex biomimetic supported lipid bilayers via vesicle fusion.Current opinion in colloid & interface science · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is growing recognition that lipid heterogeneities in cellular membranes play an important role in the distribution and functionality of membrane proteins. However, the detection and characterization of such heterogeneities at the cellular level remains challenging. Here we report on the poorly understood relationship between lipid bilayer asymmetry and membrane protein sequestering in raft-mimicking model membrane mixtures using a powerful experimental platform comprised of confocal spectroscopy XY-scan and photon-counting histogram analyses. This experimental approach is utilized to probe the domain-specific sequestering and oligomerization state of αvβ3 and α5β1 integrins in bilayers, which contain coexisting liquid-disordered/liquid-ordered (ld/lo) phase regions exclusively in the top leaflet of the bilayer (bottom leaflet contains ld phase). Comparison with previously reported integrin sequestering data in bilayer-spanning lo-ld phase separations demonstrates that bilayer asymmetry has a profound influence on αvβ3 and α5β1 sequestering behavior. For example, both integrins sequester preferentially to the lo phase in asymmetric bilayers, but to the ld phase in their symmetric counterparts. Furthermore, our data show that bilayer asymmetry significantly influences the role of native ligands in integrin sequestering.
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Registered trials
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