ArticleBiophysical journal2013
Quantitative study of the elastic modulus of loosely attached cells in AFM indentation experiments.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
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- Cell spheroid micromechanics under large deformations.Scientific reports · 2025Article
- Isocorydine Exerts Anticancer Activity by Disrupting the Energy Metabolism and Filamentous Actin Structures of Oral Squamous Carcinoma Cells.Current issues in molecular biology · 2024Article
- Ligand Binding-Induced Cellular Membrane Deformation is Correlated with the Changes in Membrane Stiffness.The journal of physical chemistry. B · 2023Article
- Hyperelastic characterization reveals proteoglycans drive the nanoscale strain-stiffening response in hyaline cartilage.Journal of biomechanics · 2023Article
- An explicit model to extract viscoelastic properties of cells from AFM force-indentation curves.iScience · 2022Article
- Recent Advances in the Label-Free Characterization of Exosomes for Cancer Liquid Biopsy: From Scattering and Spectroscopy to Nanoindentation and Nanodevices.Nanomaterials (Basel, Switzerland) · 2021Review
- Non-invasive acquisition of mechanical properties of cells via passive microfluidic mechanisms: A review.Biomicrofluidics · 2021Review
- Physiologically Relevant Mechanics of Biodegradable Polyester Nanoparticles.Nano letters · 2020Article
- Consistent apparent Young's modulus of human embryonic stem cells and derived cell types stabilized by substrate stiffness regulation promotes lineage specificity maintenance.Cell regeneration (London, England) · 2020Article
- Stiffness of HIV-1 Mimicking Polymer Nanoparticles Modulates Ganglioside-Mediated Cellular Uptake and Trafficking.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Difference in biophysical properties of cancer-initiating cells in melanoma mutated zebrafish.Journal of the mechanical behavior of biomedical materials · 2020Article
- Endothelial Glycocalyx Layer Properties and Its Ability to Limit Leukocyte Adhesion.Biophysical journal · 2020Article
- Single-Cell Proteomic Profiling Identifies Combined AXL and JAK1 Inhibition as a Novel Therapeutic Strategy for Lung Cancer.Cancer research · 2020Article
- Mechanical response of a surface of increasing hardness covered with a nonuniform polymer brush: a numerical simulation model.RSC advances · 2020Article
- Examination of the relationship between viscoelastic properties and the invasion of ovarian cancer cells by atomic force microscopy.Beilstein journal of nanotechnology · 2020Article
- Polyacrylamide Bead Sensors for in vivo Quantification of Cell-Scale Stress in Zebrafish Development.Scientific reports · 2019Article
- The cell adhesion molecule IGPR-1 is activated by and regulates responses of endothelial cells to shear stress.The Journal of biological chemistry · 2019Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When measuring the elastic (Young's) modulus of cells using AFM, good attachment of cells to a substrate is paramount. However, many cells cannot be firmly attached to many substrates. A loosely attached cell is more compliant under indenting. It may result in artificially low elastic modulus when analyzed with the elasticity models assuming firm attachment. Here we suggest an AFM-based method/model that can be applied to extract the correct Young's modulus of cells loosely attached to a substrate. The method is verified by using primary breast epithelial cancer cells (MCF-7) at passage 4. At this passage, approximately one-half of cells develop enough adhesion with the substrate to be firmly attached to the substrate. These cells look well spread. The other one-half of cells do not develop sufficient adhesion, and are loosely attached to the substrate. These cells look spherical. When processing the AFM indentation data, a straightforward use of the Hertz model results in a substantial difference of the Young's modulus between these two types of cells. If we use the model presented here, we see no statistical difference between the values of the Young's modulus of both poorly attached (round) and firmly attached (close to flat) cells. In addition, the presented model allows obtaining parameters of the brush surrounding the cells. The cellular brush observed is also statistically identical for both types of cells. The method described here can be applied to study mechanics of many other types of cells loosely attached to substrates, e.g., blood cells, some stem cells, cancerous cells, etc.
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