Evidence map›Paper›PMID 23707622›Full record

ReviewMethods (San Diego, Calif.)2013

Identifying and characterizing Hfq-RNA interactions.

M A Faner, A L Feig

Abstract readReview
In one paragraph

Review in Methods (San Diego, Calif.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Quantifying Label-Free Protein-RNA Binding Using Microscale Thermophoresis.Methods in molecular biology (Clifton, N.J.) · 2027
    Article
  2. Article
  3. Kinetic and Thermodynamic Analyses of RNA-Protein Interactions.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  4. Review
  5. Article
  6. Small RNA GadY inFrontiers in cellular and infection microbiology · 2024
    Article
  7. Article
  8. Article
  9. Regulatory RNAs: A Universal Language for Inter-Domain Communication.International journal of molecular sciences · 2020
    Review
  10. Review
  11. Bacterial Small RNAs in the GenusInternational journal of molecular sciences · 2018
    Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

M A FanerDepartment of Chemistry, Wayne State University, 5101 Cass Ave., Detroit, MI, United States.
A L Feig

Funding

Investigations of Hfq-RNA InteractionsR01GM075068 · NIGMS · WAYNE STATE UNIVERSITY · PI FEIG, ANDREW L · 2005 to 2008
$931k
NIGMS NIH HHS GM-075068NIGMS NIH HHS R01 GM075068
6 · The paper itself

Abstract

To regulate stress responses and virulence, bacteria use small regulatory RNAs (sRNAs). These RNAs can up or down regulate target mRNAs through base pairing by influencing ribosomal access and RNA decay. A large class of these sRNAs, called trans-encoded sRNAs, requires the RNA binding protein Hfq to facilitate base pairing between the regulatory RNA and its target mRNA. The resulting network of regulation is best characterized in Escherichia coli and Salmonella typhimurium, but the importance of Hfq dependent sRNA regulation is recognized in a diverse population of bacteria. In this review we present the approaches and methods used to discover Hfq binding RNAs, characterize their interactions and elucidate their functions.

Indexed as

BacteriaElectrophoretic Mobility Shift AssayGene Expression ProfilingGene Expression Regulation, BacterialHost Factor 1 ProteinImmunoprecipitationModels, MolecularProtein BindingProteomicsRNA, BacterialRNA, Small UntranslatedSELEX Aptamer TechniqueHost Factor 1 ProteinRNA, BacterialRNA, Small Untranslated2-methylnicotinic acid imidizolide5′ phosphate dependent terminator endonuclease5′RACE5′ rapid amplification of cDNA ends(ARN)(x)CLIPCLRcoimmunoprecipitationco-IPcontext likelihood of relatednesscross-linking and immunoprecipitationelectrophoretic mobility gel shift assayEMSAHfqhigh through-put sequencingHTSisothermal titration calorimetryITCLC–MSliquid chromatography–mass spectrometryMALDI-TOFMASTmatrix-assisted laser desorption/ionization time-of-flightMEMEmotif alignment and search toolmultiple em for motif elicitationNAIncRNAN-methylisatoic anhydrideNMIAProtein–RNA interactionsqRT-PCRquantitative reverse transcription polymerase chain reactionreverse transcriptionribonucleoproteinRNPRTselective 2′-hydroxyl acylation analyzed by primer extensionSELEXSHAPEsmall regulatory RNAsSPRsRNAsRNAssurface plasmon resonancesystematic evolution of ligands by exponential enrichmentTBETEXTris/Borate/EDTAwhere A is adenine, R is adenine or guanosine, and N is any nucleotide

Identifiers

PMID23707622
PMCPMC3787079

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.