ArticlePloS one2013
Regenerative capacity of old muscle stem cells declines without significant accumulation of DNA damage.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
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- Bleomycin-treated myoblasts undergo p21-associated cellular senescence and have severely impaired differentiation.GeroScience · 2024Article
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- Advanced Glycation End Products Are Retained in Decellularized Muscle Matrix Derived from Aged Skeletal Muscle.International journal of molecular sciences · 2021Article
- In vivo analysis of γH2AX+ cells in skeletal muscle from aged and obese humans.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- DNA damage in aging, the stem cell perspective.Human genetics · 2020Review
- Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation.Skeletal muscle · 2020Review
- Quantification of DNA Damage in Different Tissues in Rats with Heart Failure.Arquivos brasileiros de cardiologia · 2020Article
- NADNature metabolism · 2020Review
- Small molecule nicotinamide N-methyltransferase inhibitor activates senescent muscle stem cells and improves regenerative capacity of aged skeletal muscle.Biochemical pharmacology · 2019Article
- ---Mechanosensitivity of aged muscle stem cells.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Article
- Timed Delivery of Therapy Enhances Functional Muscle Regeneration.Advanced healthcare materials · 2017Article
- Rejuvenating Strategies for Stem Cell-Based Therapies in Aging.Cell stem cell · 2017Review
- DNA Damage Response and DNA Repair in Skeletal Myocytes From a Mouse Model of Spinal Muscular Atrophy.Journal of neuropathology and experimental neurology · 2016Article
- Ageing in relation to skeletal muscle dysfunction: redox homoeostasis to regulation of gene expression.Mammalian genome : official journal of the International Mammalian Genome Society · 2016Review
- Stem Cell-Specific Mechanisms Ensure Genomic Fidelity within HSCs and upon Aging of HSCs.Cell reports · 2015Article
- Review
- Changes in Communication between Muscle Stem Cells and their Environment with Aging.Journal of neuromuscular diseases · 2015Article
- Oxytocin is an age-specific circulating hormone that is necessary for muscle maintenance and regeneration.Nature communications · 2014Article
- Cancer stem cell theory and the warburg effect, two sides of the same coin?International journal of molecular sciences · 2014Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The performance of adult stem cells is crucial for tissue homeostasis but their regenerative capacity declines with age, leading to failure of multiple organs. In skeletal muscle this failure is manifested by the loss of functional tissue, the accumulation of fibrosis, and reduced satellite cell-mediated myogenesis in response to injury. While recent studies have shown that changes in the composition of the satellite cell niche are at least in part responsible for the impaired function observed with aging, little is known about the effects of aging on the intrinsic properties of satellite cells. For instance, their ability to repair DNA damage and the effects of a potential accumulation of DNA double strand breaks (DSBs) on their regenerative performance remain unclear. This work demonstrates that old muscle stem cells display no significant accumulation of DNA DSBs when compared to those of young, as assayed after cell isolation and in tissue sections, either in uninjured muscle or at multiple time points after injury. Additionally, there is no significant difference in the expression of DNA DSB repair proteins or globally assayed DNA damage response genes, suggesting that not only DNA DSBs, but also other types of DNA damage, do not significantly mark aged muscle stem cells. Satellite cells from DNA DSB-repair-deficient SCID mice do have an unsurprisingly higher level of innate DNA DSBs and a weakened recovery from gamma-radiation-induced DNA damage. Interestingly, they are as myogenic in vitro and in vivo as satellite cells from young wild type mice, suggesting that the inefficiency in DNA DSB repair does not directly correlate with the ability to regenerate muscle after injury. Overall, our findings suggest that a DNA DSB-repair deficiency is unlikely to be a key factor in the decline in muscle regeneration observed upon aging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.