ArticleJournal of cell science2013
ε-tubulin is essential in Tetrahymena thermophila for the assembly and stability of basal bodies.
Article in Journal of cell science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 39 citations in OpenAlex.
- Emerging Roles of Tubulin Isoforms and Their Post-Translational Modifications in Microtubule-Based Transport and Cellular Functions.Biomolecules · 2026Review
- A basal body microtubule singlet-to-doublet transition in Plasmodium male gametogenesis.Nature communications · 2025Article
- A delta-tubulin/epsilon-tubulin/Ted protein complex is required for centriole architecture.eLife · 2025Article
- Identification and bioinformatics analysis of cilia-associated gene families inFrontiers in microbiology · 2025Article
- Epsilon tubulin is an essential determinant of microtubule-based structures in male germ cells.EMBO reports · 2024Article
- Mitotic Poisons in Research and Medicine.Molecules (Basel, Switzerland) · 2020Review
- An epigenetic map of malaria parasite development from host to vector.Scientific reports · 2020Article
- The Structural and Molecular Underpinnings of Gametogenesis inFrontiers in cellular and infection microbiology · 2020Review
- Motile Cilia: Innovation and Insight From Ciliate Model Organisms.Frontiers in cell and developmental biology · 2019Review
- Review
- A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies.Nature genetics · 2018Article
- Katanin-like 2 (KATNAL2) functions in multiple aspects of haploid male germ cell development in the mouse.PLoS genetics · 2017Article
- Centriole triplet microtubules are required for stable centriole formation and inheritance in human cells.eLife · 2017Article
- The ABCs of Centriole Architecture: The Form and Function of Triplet Microtubules.Cold Spring Harbor symposia on quantitative biology · 2017Article
- Tetrahymena Poc1 ensures proper intertriplet microtubule linkages to maintain basal body integrity.Molecular biology of the cell · 2016Article
- A primer on the mouse basal body.Cilia · 2016Review
- Automated image analysis reveals the dynamic 3-dimensional organization of multi-ciliary arrays.Biology open · 2015Article
- Zeta-Tubulin Is a Member of a Conserved Tubulin Module and Is a Component of the Centriolar Basal Foot in Multiciliated Cells.Current biology : CB · 2015Article
- Kinesin-13 regulates the quantity and quality of tubulin inside cilia.Molecular biology of the cell · 2015Article
- Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Basal bodies and centrioles are conserved microtubule-based organelles the improper assembly of which leads to a number of diseases, including ciliopathies and cancer. Tubulin family members are conserved components of these structures that are integral to their proper formation and function. We have identified the ε-tubulin gene in Tetrahymena thermophila and detected the protein, through fluorescence of a tagged allele, to basal bodies. Immunoelectron microscopy has shown that ε-tubulin localizes primarily to the core microtubule scaffold. A complete genomic knockout of ε-tubulin has revealed that it is an essential gene required for the assembly and maintenance of the triplet microtubule blades of basal bodies. We have conducted site-directed mutagenesis of the ε-tubulin gene and shown that residues within the nucleotide-binding domain, longitudinal interacting domains, and C-terminal tail are required for proper function. A single amino acid change of Thr150, a conserved residue in the nucleotide-binding domain, to Val is a conditional mutation that results in defects in the spatial and temporal assembly of basal bodies as well as their stability. We have genetically separated functions for the domains of ε-tubulin and identified a novel role for the nucleotide-binding domain in the regulation of basal body assembly and stability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.