ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2013
Transgenic retinoic acid sensor lines in zebrafish indicate regions of available embryonic retinoic acid.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Nr2f1a and Isl1 repress acquisition of epicardial identity in venous atrial cardiomyocytes.Development (Cambridge, England) · 2026Article
- Stx4 is required to regulate cardiomyocyte CaHGG advances · 2022Article
- Somite morphogenesis is required for axial blood vessel formation during zebrafish embryogenesis.eLife · 2022Article
- Retinoic acid signaling restricts the size of the first heart field within the anterior lateral plate mesoderm.Developmental biology · 2021Article
- Elevated Hoxb5b Expands Vagal Neural Crest Pool and Blocks Enteric Neuronal Development in Zebrafish.Frontiers in cell and developmental biology · 2021Article
- Identifying vitamin A signaling by visualizing gene and protein activity, and by quantification of vitamin A metabolites.Methods in enzymology · 2020Article
- Recent insights on the role and regulation of retinoic acid signaling during epicardial development.Genesis (New York, N.Y. : 2000) · 2019Review
- HDAC1-mediated repression of the retinoic acid-responsive gene ripply3 promotes second heart field development.PLoS genetics · 2019Article
- Hindbrain induction and patterning during early vertebrate development.Cellular and molecular life sciences : CMLS · 2019Review
- In Silico Identification and Experimental Validation of (-)-Muqubilin A, a Marine Norterpene Peroxide, as PPARα/γ-RXRα Agonist and RARα Positive Allosteric Modulator.Marine drugs · 2019Article
- Collagen COL22A1 maintains vascular stability and mutations inDisease models & mechanisms · 2018Article
- Cyp26 Enzymes Facilitate Second Heart Field Progenitor Addition and Maintenance of Ventricular Integrity.PLoS biology · 2016Article
- Retinoic acid and meiosis induction in adult versus embryonic gonads of medaka.Scientific reports · 2016Article
- Retinoic acid-induced premature osteoblast-to-preosteocyte transitioning has multiple effects on calvarial development.Development (Cambridge, England) · 2016Article
- Retinoic Acid Signaling Coordinates Macrophage-Dependent Injury and Repair after AKI.Journal of the American Society of Nephrology : JASN · 2016Article
- Visualizing retinoic acid morphogen gradients.Methods in cell biology · 2016Article
- Excessive feedback of Cyp26a1 promotes cell non-autonomous loss of retinoic acid signaling.Developmental biology · 2015Article
- Zic1 controls placode progenitor formation non-cell autonomously by regulating retinoic acid production and transport.Nature communications · 2015Article
- Retinoic acid plays an evolutionarily conserved and biphasic role in pancreas development.Developmental biology · 2014Article
- Depletion of retinoic acid receptors initiates a novel positive feedback mechanism that promotes teratogenic increases in retinoic acid.PLoS genetics · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundRetinoic acid (RA) signaling plays a critical role in vertebrate development. Transcriptional reporters of RA signaling in zebrafish, thus far, have not reflected the broader availability of embryonic RA, necessitating additional tools to enhance our understanding of the spatial and temporal activity of RA signaling in vivo.
resultsWe have generated novel transgenic RA sensors in which a RA receptor (RAR) ligand-binding domain (RLBD) is fused to the Gal4 DNA-binding domain (GDBD) or a VP16-GDBD (VPBD) construct. Stable transgenic lines expressing these proteins when crossed with UAS reporter lines are responsive to RA. Interestingly, the VPBD RA sensor is significantly more sensitive than the GDBD sensor and demonstrates there may be almost ubiquitous availability of RA within the early embryo. Using confocal microscopy to compare the expression of the GDBD RA sensor to our previously established RA signaling transcriptional reporter line, Tg(12XRARE:EGFP), illustrates these reporters have significant overlap, but that expression from the RA sensor is much broader. We also identify previously unreported domains of expression for the Tg(12XRARE:EGFP) line.
conclusionsOur novel RA sensor lines will be useful and complementary tools for studying RA signaling during development and anatomical structures independent of RA signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.