Evidence map›Paper›PMID 23703692›Full record

ArticleStem cell reviews and reports2013

SCF promotes dental pulp progenitor migration, neovascularization, and collagen remodeling - potential applications as a homing factor in dental pulp regeneration.

Shuang Pan, Smit Dangaria, Gokul Gopinathan, Xiulin Yan, Xuanyu Lu, Antonia Kolokythas, Yumei Niu, Xianghong Luan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reviews and reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
2.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 62 citations in OpenAlex.

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  7. Influencing factors of pulp-dentin complex regeneration and related biological strategies.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Shuang PanSchool of Dentistry, Department of Endodontics, Harbin Medical University, Harbin, China.
Smit Dangaria
Gokul Gopinathan
Xiulin Yan
Xuanyu Lu
Antonia Kolokythas
Yumei Niu
Xianghong Luan
University of Illinois Chicago · USHarbin Medical University · CN

Funding

Small Molecule Microenvironment Design for Craniofacial Bone Regeneration and EngineeringR01DE019463 · NIDCR · UNIVERSITY OF ROCHESTER · PI LUAN, XIANGHONG · 2009 to 2021
$2.8M
NIDCR NIH HHS DE019463NIDCR NIH HHS R01 DE019463
6 · The paper itself

Abstract

Stem cell factor (SCF) is a powerful chemokine that binds to the c-Kit receptor CD117 and has shown promise as a homing agent capable of progenitor cell recruitment. In the present study we have documented high levels of both SCF and its receptor c-Kit in differentiating dental pulp (DP) cells and in the sub-odontoblastic layer of Höhl. In vitro studies using human DP progenitors revealed a significant increase in cell proliferation after100 nM SCF application, explained by a 2-fold upregulation in cyclin D3 and FGF2 cell cycle regulators, and a 7-fold increase in CDK4 expression. DP cell migration in the presence of SCF was up-regulated 2.7-fold after a 24 h culture period, and this effect was accompanied by cytoskeletal rearrangement, a 1.5-fold increase in polymeric F-actin over G-actin, and a 1.8-fold increase in RhoA expression. Explaining the signaling effect of SCF on DP migration, PI3K/Akt and MEK/ERK pathway inhibitors were demonstrated to significantly reduce DP cell migration, while SCF alone doubled the number of migrated cells. ERK and AKT phosphorylation were dramatically upregulated already 3-5 min after SCF addition to the culture medium and declined thereafter, classifying SCF as a fast acting chemokine. When applied as an agent to promote tissue regeneration in subcutaneously implanted collagen sponges, SCF resulted in a 7-fold increase in the cell number in the implanted tissue construct, a more than 9-fold increase in capillaries, as well as collagen sponge remodeling and collagen fiber neogenesis. Together, these studies demonstrate the suitability of SCF as a potent aid in the regeneration of dental pulp and other mesenchymal tissues, capable of inducing cell homing, angiogenesis, and tissue remodeling.

Indexed as

AdolescentAnimalsBlotting, WesternCell MovementCells, CulturedChildCollagenCyclin D3Cyclin-Dependent Kinase 4Dental PulpExtracellular Signal-Regulated MAP KinasesGene Expression Regulation, DevelopmentalHumansMesenchymal Stem CellsMesenchymal Stem Cell TransplantationMiceCollagenCyclin D3Cyclin-Dependent Kinase 4Extracellular Signal-Regulated MAP KinasesPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-kitStem Cell Factor

Identifiers

PMID23703692
PMCPMC5023022
OpenAlexW2021926216

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.