ArticleGastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association2014
Helicobacter pylori urease and flagellin alter mucin gene expression in human gastric cancer cells.
Article in Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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19 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- The association of fungal infection in cervical cancer: a systematic review and meta-analysis.BMC women's health · 2026Pooled it
- Potential Efficacy of Propolis in TreatingNutrients · 2025Review
- Relationship between mucin gene polymorphisms and different types of gallbladder stones.BMC medical genomics · 2025Article
- Recognizing the biological barriers and pathophysiological characteristics of the gastrointestinal tract for the design and application of nanotherapeutics.Drug delivery · 2024Review
- The chimeric UreB, FliD and Omp18 proteins for a sensitive and specific diagnosis ofIranian journal of microbiology · 2024Article
- Effect ofJournal of enzyme inhibition and medicinal chemistry · 2023Article
- Review
- Integrated analysis of necroptosis-related genes for evaluating immune infiltration and colon cancer prognosis.Frontiers in immunology · 2022Article
- Complete Genome Sequences of Leclercia sp. W6 and W17 Isolated from a Gastric Cancer Patient.Current microbiology · 2020Article
- Bioinformatics analysis of small RNAs in Helicobacter pylori and the role of NAT‑67 under tinidazole treatment.Molecular medicine reports · 2020Article
- Upregulation of MUC5AC production and deposition of LEWIS determinants by HELICOBACTER PYLORI facilitate gastric tissue colonization and the maintenance of infection.Journal of biomedical science · 2019Article
- The Relationship between Toll-like Receptors andJournal of immunology research · 2019Review
- Effects of the Helicobacter pylori Virulence Factor CagA and Ammonium Ion on Mucins in AGS Cells.Yonsei medical journal · 2018Article
- Ramifications of secreted mucin MUC5AC in malignant journey: a holistic view.Carcinogenesis · 2018Review
- Transfection of theOncology letters · 2018Article
- A New Role forFrontiers in microbiology · 2017Article
- The Impact ofFrontiers in microbiology · 2017Article
- Role of Toll-like receptors in Helicobacter pylori infection and immunity.World journal of gastrointestinal pathophysiology · 2014Review
- Mucin 1 gene (MUC1) and gastric-cancer susceptibility.International journal of molecular sciences · 2014Article
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7 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundHelicobacter pylori (Hp), which is one of the causative agents in human gastric adenocarcinoma, is known to interact with mucous gel and alter mucin gene expression. The aim of this work was to study, using an in vitro model of cell infection, the effects of urease, flagellin, and CagA virulence factors on the regulation of the four 11p15 mucin genes (MUC2, MUC5AC, MUC5B, and MUC6).
methodsKATO-III and AGS gastric cancer cells were infected for 1, 3 or 6 h with Hp wild-type strains (ATCC 43504, N6, and SS1) or corresponding isogenic mutants deficient for urease subunit B, flagellin subunit A, and CagA. mRNA levels of MUC2, MUC5B, MUC5AC and MUC6 were assessed by RT-PCR, and functional activity of their promoters was measured by transient transfection assays.
resultsInfection of KATO-III cells with Hp wild-type strains resulted in an early (at 1 h) transient expression of MUC2, MUC5AC, and MUC6 mRNA concomitant with those of interleukin (IL)-1β, IL-8, and TNF-α cytokines. In these cells, the UreB(-) isogenic mutant induced strong activation of MUC5AC expression, and UreB-responsive elements were located in the -486/-1 region of the promoter. FlaA(-) and CagA(-) mutants had no effect on mucin gene mRNA levels in KATO-III cells. In AGS cells, Hp-responsive elements were identified in all promoters, and overexpression of NF-κB induced upregulation of MUC5AC promoter activity when infected with the UreB(-) isogenic mutant.
conclusionThese results indicate that Hp infection of gastric cancer cells alters 11p15 mucin gene transcription and that MUC5AC downregulation is mediated by urease virulence factor.
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