ArticleJournal of clinical pathology1990
Detection of messenger RNA using a digoxigenin end labelled oligodeoxynucleotide probe.
Article in Journal of clinical pathology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Mutant activated FGFR3 impairs endochondral bone growth by preventing SOX9 downregulation in differentiating chondrocytes.Human molecular genetics · 2015Article
- Gene Expression Profiling during Murine Tooth Development.Frontiers in genetics · 2012Article
- Rapid in situ codetection of noncoding RNAs and proteins in cells and formalin-fixed paraffin-embedded tissue sections without protease treatment.Nature protocols · 2010Article
- Simultaneous fluorescence in situ hybridization of mRNA and rRNA in environmental bacteria.Applied and environmental microbiology · 2004Article
- Chromogenic in situ hybridization for alpha6beta4 integrin in breast cancer: correlation with protein expression.The Journal of molecular diagnostics : JMD · 2004Article
- Expression of somatostatin mRNA and peptides in C-cell tumours of the thyroid gland in Han Wistar rats.International journal of experimental pathology · 2004Article
- In situ production of gamma interferon, interleukin-4, and tumor necrosis factor alpha mRNA in human lung tuberculous granulomas.Infection and immunity · 2000Article
- Detection of different mRnas expressed in the thyro-parathyroid complex of the rat by in situ hybridization using digoxigenin-labelled oligonucleotide probes.The Histochemical journal · 2000Article
- Review
- Differential expression of CD32 isoforms following alloactivation of human T cells.Immunology · 1997Article
- In situ hybridization studies of metalloproteinases 2 and 9 and TIMP-1 and TIMP-2 expression in human prostate cancer.Clinical & experimental metastasis · 1997Article
- A one-day double-labelling technique for tissue specimens: immunogold-silver staining for in situ hybridization combined with alkaline phosphatase-anti-alkaline phosphatase (APAAP) immunohistochemistry for antigens.The Histochemical journal · 1996Article
- The detection of the mRNAs of procollagen types I, II and III in human fetal fingers by in situ hybridization using digoxigenin-labelled oligonucleotide probes.The Histochemical journal · 1995Article
- Kinetic microphotometric evaluation of in situ hybridization for mRNA of slow myosin heavy chain in type I and C fibres of rabbit muscle.Histochemistry · 1994Article
- Detection of tumour necrosis factor alpha in sarcoidosis and tuberculosis granulomas using in situ hybridisation.Journal of clinical pathology · 1994Article
- Fine-structural localization of proenkephalin mRNAs in the hypothalamic magnocellular dorsal nucleus of the guinea pig: a comparison of radioisotopic and enzymatic in situ hybridization methods at the light- and electron-microscopic levels.Cell and tissue research · 1993Article
- Demonstration of mRNA using digoxigenin labelled oligonucleotide probes for in situ hybridisation in formamide free conditions.Journal of clinical pathology · 1993Article
- In situ detection of human Ig light-chain mRNA on formalin-fixed and paraffin-embedded tissue sections using digoxigenin-labelled RNA probes.The Histochemical journal · 1993Article
- Oestrogen receptor protein and mRNA in adenocarcinoma of the uterine cervix.British journal of cancer · 1992Article
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A synthetic oligodeoxynucleotide sequence complementary to the mRNA for the adrenocorticotrophin (ACTH) precursor pro-opiomelanocortin (POMC) was end labelled using digoxigenin. The probe was used to detect POMC mRNA both on nitrocellulose filters and by non-isotopic in situ hybridisation (NISH) in tissue sections. Digoxigenin was identified using anti-digoxigenin alkaline phosphatase. The model system examined was the rat pituitary gland. Removal of both adrenal glands and dexamethasone administration were used to change the concentrations of POMC mRNA in the rat anterior lobe. The labelled probe reacted with a single band of appropriate molecular weight in Northern blot analysis. The distribution of signal in tissue sections and the changes induced by experimental manipulation were as predicted. The results indicate that this method of NISH will prove useful in the detection of specific messenger RNAs in tissue sections of buffered, formalin fixed, paraffin wax embedded material.
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