ArticleNature protocols2013
High-resolution membrane capacitance measurements for the study of exocytosis and endocytosis.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
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- Astrocyte arborization enhances CaGlia · 2021Article
- Impact of P2XInternational journal of molecular sciences · 2021Article
- Exocytotic fusion pore under stress.Cell stress · 2020Review
- Exocytosis of large-diameter lysosomes mediates interferon γ-induced relocation of MHC class II molecules toward the surface of astrocytes.Cellular and molecular life sciences : CMLS · 2020Article
- TPC1 deficiency or blockade augments systemic anaphylaxis and mast cell activity.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Astroglial Mechanisms of Ketamine Action Include Reduced Mobility of Kir4.1-Carrying Vesicles.Neurochemical research · 2020Review
- An isogenic hiPSC-derived BBB-on-a-chip.Biomicrofluidics · 2019Article
- Membrane capacitance recordings resolve dynamics and complexity of receptor-mediated endocytosis in Wnt signalling.Scientific reports · 2019Article
- Optical Tracking of Nanometer-Scale Cellular Membrane Deformation Associated with Single Vesicle Release.ACS sensors · 2019Article
- Astrocyte Specific Remodeling of Plasmalemmal Cholesterol Composition by Ketamine Indicates a New Mechanism of Antidepressant Action.Scientific reports · 2019Article
- Gliocrine System: Astroglia as Secretory Cells of the CNS.Advances in experimental medicine and biology · 2019Review
- Astroglial vesicular network: evolutionary trends, physiology and pathophysiology.Acta physiologica (Oxford, England) · 2018Review
- Sphingomimetic multiple sclerosis drug FTY720 activates vesicular synaptobrevin and augments neuroendocrine secretion.Scientific reports · 2017Article
- The Instrumentation of a Microfluidic Analyzer Enabling the Characterization of the Specific Membrane Capacitance, Cytoplasm Conductivity, and Instantaneous Young's Modulus of Single Cells.International journal of molecular sciences · 2017Article
- Ketamine Inhibits ATP-Evoked Exocytotic Release of Brain-Derived Neurotrophic Factor from Vesicles in Cultured Rat Astrocytes.Molecular neurobiology · 2016Article
- Dominant negative SNARE peptides stabilize the fusion pore in a narrow, release-unproductive state.Cellular and molecular life sciences : CMLS · 2016Article
- CRACM3 regulates the stability of non-excitable exocytotic vesicle fusion pores in a Ca(2+)-independent manner via molecular interaction with syntaxin4.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to understand exocytosis and endocytosis, it is necessary to study these processes directly. An elegant way to do this is by measuring plasma membrane capacitance (C(m)), a parameter proportional to cell surface area, the fluctuations of which are due to fusion and fission of secretory and other vesicles. Here we describe protocols that enable high-resolution C(m) measurements in macroscopic and microscopic modes. Macroscopic mode, performed in whole-cell configuration, is used for measuring bulk C(m) changes in the entire membrane area, and it enables the introduction of exocytosis stimulators or inhibitors into the cytosol through the patch pipette. Microscopic mode, performed in cell-attached configuration, enables measurements of C(m) with attofarad resolution and allows characterization of fusion pore properties. Although we usually apply these protocols to primary pituitary cells and astrocytes, they can be adapted and used for other cell types. After initial hardware setup and culture preparation, several C(m) measurements can be performed daily.
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