ArticleCell calcium2013
Role of polyhydroxybutyrate in mitochondrial calcium uptake.
Article in Cell calcium, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Review
- Inorganic polyphosphate and the regulation of mitochondrial physiology.Biochemical Society transactions · 2023Article
- The proteomic effects of ketone bodies: implications for proteostasis and brain proteinopathies.Frontiers in molecular neuroscience · 2023Review
- Review
- Review
- Inorganic polyphosphate is required for sustained free mitochondrial calcium elevation, following calcium uptake.Cell calcium · 2020Article
- Pharmacological Sequestration of Mitochondrial Calcium Uptake Protects Neurons Against Glutamate Excitotoxicity.Molecular neurobiology · 2019Article
- Carbonic anhydrase inhibition selectively prevents amyloid β neurovascular mitochondrial toxicity.Aging cell · 2018Article
- Rapid frequency-dependent changes in free mitochondrial calcium concentration in rat cardiac myocytes.The Journal of physiology · 2017Article
- Mitochondrial CaJournal of bioenergetics and biomembranes · 2017Review
- Modulation of mitochondrial ion transport by inorganic polyphosphate - essential role in mitochondrial permeability transition pore.Journal of bioenergetics and biomembranes · 2017Article
- Contribution of inorganic polyphosphate towards regulation of mitochondrial free calcium.Biochimica et biophysica acta · 2016Article
- Inorganic polyphosphate (polyP) as an activator and structural component of the mitochondrial permeability transition pore.Biochemical Society transactions · 2016Review
- Role of β-hydroxybutyrate, its polymer poly-β-hydroxybutyrate and inorganic polyphosphate in mammalian health and disease.Frontiers in physiology · 2014Review
- Polyhydroxybutyrate targets mammalian mitochondria and increases permeability of plasmalemmal and mitochondrial membranes.PloS one · 2013Article
- Calculation of ion currents across the inner membrane of functionally intact mitochondria.Channels (Austin, Tex.)Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 4 countries.
Funding
Abstract
Polyhydroxybutyrate (PHB) is a biological polymer which belongs to the class of polyesters and is ubiquitously present in all living organisms. Mammalian mitochondrial membranes contain PHB consisting of up to 120 hydroxybutyrate residues. Roles played by PHB in mammalian mitochondria remain obscure. It was previously demonstrated that PHB of the size similar to one found in mitochondria mediates calcium transport in lipid bilayer membranes. We hypothesized that the presence of PHB in mitochondrial membrane might play a significant role in mitochondrial calcium transport. To test this, we investigated how the induction of PHB hydrolysis affects mitochondrial calcium transport. Mitochondrial PHB was altered enzymatically by targeted expression of bacterial PHB hydrolyzing enzyme (PhaZ7) in mitochondria of mammalian cultured cells. The expression of PhaZ7 induced changes in mitochondrial metabolism resulting in decreased mitochondrial membrane potential in HepG2 but not in U87 and HeLa cells. Furthermore, it significantly inhibited mitochondrial calcium uptake in intact HepG2, U87 and HeLa cells stimulated by the ATP or by the application of increased concentrations of calcium to the digitonin permeabilized cells. Calcium uptake in PhaZ7 expressing cells was restored by mimicking calcium uniporter properties with natural electrogenic calcium ionophore - ferutinin. We propose that PHB is a previously unrecognized important component of the mitochondrial calcium uptake system.
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Registered trials
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