ArticleHuman genetics1990
Chromosomal in situ suppression hybridization of human gonosomes and autosomes and its use in clinical cytogenetics.
Article in Human genetics, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 88 citations in OpenAlex.
- American College of Medical Genetics guideline on the cytogenetic evaluation of the individual with developmental delay or mental retardation.Genetics in medicine : official journal of the American College of Medical GeneticsGuideline
- Congenital heart disease and chromossomopathies detected by the karyotype.Revista paulista de pediatria : orgao oficial da Sociedade de Pediatria de Sao Paulo · 2014Article
- Recollections of a scientific journey published in human genetics: from chromosome territories to interphase cytogenetics and comparative genome hybridization.Human genetics · 2014Article
- Black rat ( Rattus rattus) genomic variability characterized by chromosome painting.Mammalian genome : official journal of the International Mammalian Genome Society · 2002Article
- Spatial relationship between transcription sites and chromosome territories.The Journal of cell biology · 1999Article
- Multiplex-FISH for pre- and postnatal diagnostic applications.American journal of human genetics · 1999Article
- Molecular, cytogenetic, and clinical characterisation of six XX males including one prenatal diagnosis.Journal of medical genetics · 1998Article
- Three-dimensional reconstruction of painted human interphase chromosomes: active and inactive X chromosome territories have similar volumes but differ in shape and surface structure.The Journal of cell biology · 1996Article
- Chromosome painting defines genomic rearrangements between red howler monkey subspecies.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1996Article
- Value of chromosome painting in determining the chromosomal outcome in offspring of a 12;16 translocation carrier.Journal of medical genetics · 1994Article
- Elucidation of structural abnormalities of the X chromosome using fluorescence in situ hybridisation with a Y chromosome painting probe.Journal of medical genetics · 1994Article
- Characterization of supernumerary ring marker chromosomes by fluorescence in situ hybridization (FISH).American journal of human genetics · 1993Article
- The origin of cytologically unidentifiable chromosome abnormalities: six cases ascertained by targeted chromosome-band painting.Human genetics · 1993Article
- Five year study of prenatal testing for Huntington's disease: demand, attitudes, and psychological assessment.Journal of medical genetics · 1993Article
- Characterization of de novo duplications in eight patients by using fluorescence in situ hybridization with chromosome-specific DNA libraries.American journal of human genetics · 1993Article
- Detection of complete and partial chromosome gains and losses by comparative genomic in situ hybridization.Human genetics · 1993Article
- Complete characterization of a large marker chromosome by reverse and forward chromosome painting.Human genetics · 1992Article
- Rapid detection of chromosome aneuploidies in uncultured amniocytes by using fluorescence in situ hybridization (FISH).American journal of human genetics · 1992Article
- Article
- Reverse chromosome painting: a method for the rapid analysis of aberrant chromosomes in clinical cytogenetics.Journal of medical genetics · 1992Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA libraries from sorted human gonosomes were used selectively to stain the X and Y chromosomes in normal and aberrant cultured human cells by chromosomal in situ suppression (CISS-) hybridization. The entire X chromosome was stained in metaphase spreads. Interphase chromosome domains of both the active and inactive X were clearly delineated. CISS-hybridization of the Y chromosome resulted in the specific decoration of the euchromatic part (Ypter-q11), whereas the heterochromatic part (Yq12) remained unlabeled. The stained part of the Y chromosome formed a compact domain in interphase nuclei. This approach was applied to amniotic fluid cells containing a ring chromosome of unknown origin (47,XY: +r). The ring chromosome was not stained by library probes from the gonosomes, thereby suggesting its autosomal origin. The sensitivity of CISS-hybridization was demonstrated by the detection of small translocations and fragments in human lymphocyte metaphase spreads after irradiation with 60Co-gamma-rays. Lymphocyte cultures from two XX-males were investigated by CISS-hybridization with Y-library probes. In both cases, metaphase spreads demonstrated a translocation of Yp-material to the short arm of an X chromosome. The translocated Y-material could also be demonstrated directly in interphase nuclei. CISS-hybridization of autosomes 7 and 13 was used for prenatal diagnosis in a case with a known balanced translocation t(7:13) in the father. The same translocation was observed in amniotic fluid cells from the fetus. Specific staining of the chromosomes involved in such translocations will be particularly important, in the future, in cases that cannot be solved reliably by conventional chromosome banding alone.
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