ArticlePloS one2013
Reference genes to study herbicide stress response in Lolium sp.: up-regulation of P450 genes in plants resistant to acetolactate-synthase inhibitors.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 74 citations in OpenAlex.
- Subgenomic Distribution and Herbicide Cross-Resistance ofPlant direct · 2026Article
- Article
- A high diversity of non-target site resistance mechanisms to acetolactate-synthase (ALS) inhibiting herbicides has evolved within and among field populations of common ragweed (Ambrosia artemisiifolia L.).BMC plant biology · 2023Article
- Assessment of ACC and P450 Genes Expression in Wild Oat (Avena ludoviciana) in Different Tissues Under Herbicide Application.Biochemical genetics · 2023Article
- Cytochrome P450 Gene Families: Role in Plant Secondary Metabolites Production and Plant Defense.Journal of xenobiotics · 2023Review
- Transcriptomic analysis of Amaranthus retroflex resistant to PPO-inhibitory herbicides.PloS one · 2023Article
- Expression of a Cytochrome P450 Gene from Bermuda GrassPlants (Basel, Switzerland) · 2022Article
- Expression Pattern of Entire Cytochrome P450 Genes and Response of Defenses in a Metabolic-Herbicide-Resistant Biotype ofFrontiers in plant science · 2022Article
- Expression stability of internal reference gene in response to Trichoderma polysporum infection in Avena fatua L.Current genetics · 2021Article
- The analysis of reference genes expression stability in susceptible and resistant Apera spica-venti populations under herbicide treatment.Scientific reports · 2021Article
- Proteomic Profile of Glyphosate-Resistant Soybean under Combined Herbicide and Drought Stress Conditions.Plants (Basel, Switzerland) · 2021Article
- Diversified Resistance Mechanisms in Multi-ResistantFrontiers in plant science · 2020Article
- Reference gene selection for quantitative real-time PCR (qRT-PCR) expression analysis in Galium aparine L.PloS one · 2020Article
- Validation of reference genes for qRT-PCR data normalisation in lentil (Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Validation of reference genes for RT-qPCR studies inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Substrate specificity and safener inducibility of the plant UDP-glucose-dependent family 1 glycosyltransferase super-family.Plant biotechnology journal · 2018Article
- Multiple mechanisms are involved in new imazamox-resistant varieties of durum and soft wheat.Scientific reports · 2017Article
- Selection of relatively exact reference genes for gene expression studies in goosegrass (Eleusine indica) under herbicide stress.Scientific reports · 2017Article
- Molecular responses of genetically modified maize to abiotic stresses as determined through proteomic and metabolomic analyses.PloS one · 2017Article
- Transcriptome Profiling to Identify Genes Involved in Mesosulfuron-Methyl Resistance inFrontiers in plant science · 2017Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Variation in the expression of numerous genes is at the basis of plant response to environmental stresses. Non-target-site-based resistance to herbicides (NTSR), the major threat to grass weed chemical control, is governed by a subset of the genes involved in herbicide stress response. Quantitative PCR assays allowing reliable comparison of gene expression are thus key to identify genes governing NTSR. This work aimed at identifying a set of reference genes with a stable expression to be used as an internal standard for the normalisation of quantitative PCR data in studies investigating NTSR to herbicides inhibiting acetolactate synthase (ALS) in the major grass weed Lolium sp. Gene expression stability was assessed in plants resistant or sensitive to two ALS inhibitors, subjected or not to herbicide stress. Using three complementary approaches implemented in the programs BestKeeper, NormFinder and geNorm, cap-binding protein, glyceraldehyde-3-phosphate-dehydrogenase and ubiquitin were identified as the most suitable reference genes. This reference gene set can probably be used to study herbicide response in other weed species. It was used to compare the expression of the genes encoding two herbicide target enzymes (ALS and acetyl-coenzyme A carboxylase) and five cytochromes P450 (CYP) with potential herbicide-degrading activity between plants resistant or sensitive to ALS inhibitors. Overall, herbicide application enhanced CYP gene expression. Constitutive up-regulation of all CYP genes observed in resistant plants compared to sensitive plants suggested enhanced secondary metabolism in the resistant plants. Comprehensive transcriptome studies associated to gene expression analyses using the reference gene set validated here are required to unravel NTSR genetic determinants.
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