ArticleScience signaling2013
Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Article in Science signaling, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Recurrent innovation of protein-protein interactions in the Drosophila piRNA pathway.The EMBO journal · 2026Article
- Protein-Ligand Interactions in Scarcity: The Stringent Response from Bacteria to Metazoa, and the Unanswered Questions.International journal of molecular sciences · 2023Review
- A comprehensive SARS-CoV-2-human protein-protein interactome network identifies pathobiology and host-targeting therapies for COVID-19.Research square · 2022Article
- Computational Network Inference for Bacterial Interactomics.mSystems · 2022Review
- Are transient protein-protein interactions more dispensable?PLoS computational biology · 2022Article
- A 3D structural SARS-CoV-2-human interactome to explore genetic and drug perturbations.Nature methods · 2021Article
- Mutation Edgotype Drives Fitness Effect in Human.Frontiers in bioinformatics · 2021Article
- A massively parallel barcoded sequencing pipeline enables generation of the first ORFeome and interactome map for rice.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.Journal of proteomics · 2020Article
- Effect of dietary fat and sucrose consumption on cardiac fibrosis in mice and rhesus monkeys.JCI insight · 2019Article
- Estimating dispensable content in the human interactome.Nature communications · 2019Article
- Latent Model-Based Clustering for Biological Discovery.iScience · 2019Article
- Resurrected Protein Interaction Networks Reveal the Innovation Potential of Ancient Whole-Genome Duplication.The Plant cell · 2018Article
- An inter-species protein-protein interaction network across vast evolutionary distance.Molecular systems biology · 2016Article
- A Cross-Species Study of PI3K Protein-Protein Interactions Reveals the Direct Interaction of P85 and SHP2.Scientific reports · 2016Article
- A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.Cell · 2016Article
- Toxicity and SidJ-Mediated Suppression of Toxicity Require Distinct Regions in the SidE Family of Legionella pneumophila Effectors.Infection and immunity · 2015Article
- ENCAPP: elastic-net-based prognosis prediction and biomarker discovery for human cancers.BMC genomics · 2015Article
- Article
- A massively parallel pipeline to clone DNA variants and examine molecular phenotypes of human disease mutations.PLoS genetics · 2014Article
Corrections and comments
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Authors and funding
16 authors.
Funding
Abstract
The fission yeast Schizosaccharomyces pombe has more metazoan-like features than the budding yeast Saccharomyces cerevisiae, yet it has similarly facile genetics. We present a large-scale verified binary protein-protein interactome network, "StressNet," based on high-throughput yeast two-hybrid screens of interacting proteins classified as part of stress response and signal transduction pathways in S. pombe. We performed systematic, cross-species interactome mapping using StressNet and a protein interactome network of orthologous proteins in S. cerevisiae. With cross-species comparative network studies, we detected a previously unidentified component (Snr1) of the S. pombe mitogen-activated protein kinase Sty1 pathway. Coimmunoprecipitation experiments showed that Snr1 interacted with Sty1 and that deletion of snr1 increased the sensitivity of S. pombe cells to stress. Comparison of StressNet with the interactome network of orthologous proteins in S. cerevisiae showed that most of the interactions among these stress response and signaling proteins are not conserved between species but are "rewired"; orthologous proteins have different binding partners in both species. In particular, transient interactions connecting proteins in different functional modules were more likely to be rewired than conserved. By directly testing interactions between proteins in one yeast species and their corresponding binding partners in the other yeast species with yeast two-hybrid assays, we found that about half of the interactions that are traditionally considered "conserved" form modified interaction interfaces that may potentially accommodate novel functions.
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