ArticleAnalytical chemistry2013
Fast top-down intact protein characterization with capillary zone electrophoresis-electrospray ionization tandem mass spectrometry.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 62 citations in OpenAlex.
- Dual-Laser Laser-Induced Liquid Bead Ion Desorption Mass Spectrometry: A New Approach for Adjustable Softness and Higher Sensitivity.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- Capillary Electrophoresis-Mass Spectrometry for Top-Down Proteomics.Annual review of analytical chemistry (Palo Alto, Calif.) · 2025Review
- Top-down proteomics.Nature reviews. Methods primers · 2024Article
- Top-Down Proteomics and the Challenges of True Proteoform Characterization.Journal of proteome research · 2023Review
- Considerations for defining +80 Da mass shifts in mass spectrometry-based proteomics: phosphorylation and beyond.Chemical communications (Cambridge, England) · 2023Review
- CE-MS for Proteomics and Intact Protein Analysis.Advances in experimental medicine and biology · 2021Article
- Capillary Zone Electrophoresis-Tandem Mass Spectrometry with Activated Ion Electron Transfer Dissociation for Large-scale Top-down Proteomics.Journal of the American Society for Mass Spectrometry · 2019Article
- Capillary zone electrophoresis-mass spectrometry for top-down proteomics.Trends in analytical chemistry : TRAC · 2019Article
- Article
- [Comparing nanoflow reversed-phase liquid chromatography-tandem mass spectrometry and capillary zone electrophoresis-tandem mass spectrometry for top-down proteomics].Se pu = Chinese journal of chromatography · 2019Article
- Large-Scale Qualitative and Quantitative Top-Down Proteomics Using Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry with Nanograms of Proteome Samples.Journal of the American Society for Mass Spectrometry · 2019Article
- Low-Cost Localized Surface Plasmon Resonance Biosensing Platform with a Response Enhancement for Protein Detection.Nanomaterials (Basel, Switzerland) · 2019Article
- Capillary zone electrophoresis-tandem mass spectrometry using ultraviolet photodissociation (213 nm) for large-scale top-down proteomics.Analytical methods : advancing methods and applications · 2019Article
- Top-down mass spectrometry of intact phosphorylated β-casein: Correlation between the precursor charge state and internal fragments.Journal of mass spectrometry : JMS · 2019Article
- Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry.Journal of visualized experiments : JoVE · 2018Article
- Deep Top-Down Proteomics Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry: Identification of 5700 Proteoforms from the Escherichia coli Proteome.Analytical chemistry · 2018Article
- Top-Down Proteomics: Ready for Prime Time?Analytical chemistry · 2018Review
- Single-Shot Top-Down Proteomics with Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry for Identification of Nearly 600 Escherichia coli Proteoforms.Analytical chemistry · 2017Article
- The Mechanism Behind Top-Down UVPD Experiments: Making Sense of Apparent Contradictions.Journal of the American Society for Mass Spectrometry · 2017Article
- A Comprehensive Guide for Performing Sample Preparation and Top-Down Protein Analysis.Proteomes · 2017Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Capillary zone electrophoresis (CZE)-electrospray ionization tandem mass spectrometry (ESI-MS/MS) was applied for rapid top-down intact protein characterization. A mixture containing four model proteins (cytochrome c, myoglobin, bovine serum albumin (BSA), and β-casein) was used as the sample. The CZE-ESI-MS system was first evaluated with the mixture. The four model proteins and five impurities were baseline-separated within 12 min. The limits of detection [signal-to-noise ratio (S/N) = 3] of the four model proteins ranged from 20 (cytochrome c) to 800 amol (BSA). The relative standard deviations of migration time and intensity for the four model proteins were less than 3% and 30%, respectively, in quintuplicate runs. CZE-ESI-MS/MS was then applied for top-down characterization of the mixture. Three of the model proteins (all except BSA) and an impurity (bovine transthyretin) were confidently identified by database searching of the acquired tandem spectra from protein fragmentation. Modifications including phosphorylation, N-terminal acetylation, and heme group binding were identified.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.