ArticleCell proliferation2013
Overexpression of hCLP46 enhances Notch activation and regulates cell proliferation in a cell type-dependent manner.
Article in Cell proliferation, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- The glycosyltransferase POGLUT1 regulates muscle stem cell development and maintenance in mice.PLoS genetics · 2025Article
- Glycosylation in Cancer.Handbook of experimental pharmacology · 2025Review
- The glycosyltransferase POGLUT1 regulates muscle stem cell development and maintenance in mice.bioRxiv : the preprint server for biology · 2024Article
- Transcriptionally imprinted glycomic signatures of acute myeloid leukemia.Cell & bioscience · 2023Article
- Structure, function, and pathology of protein O-glucosyltransferases.Cell death & disease · 2021Review
- Multifaceted regulation of Notch signaling by glycosylation.Glycobiology · 2021Review
- Other Types of Glycosylation.Advances in experimental medicine and biology · 2021Article
- When Glycosylation Meets Blood Cells: A Glance of the Aberrant Glycosylation in Hematological Malignancies.Reviews of physiology, biochemistry and pharmacology · 2021Review
- Multiple Roles of Glycans in Hematological Malignancies.Frontiers in oncology · 2018Review
- miR-134: A Human Cancer Suppressor?Molecular therapy. Nucleic acids · 2017Review
- High-density genotyping of immune-related loci identifies new SLE risk variants in individuals with Asian ancestry.Nature genetics · 2016Article
- hCLP46 increases Smad3 protein stability via inhibiting its ubiquitin-proteasomal degradation.Protein & cell · 2015Article
- Protein O-Glucosyltransferase 1 (POGLUT1) Promotes Mouse Gastrulation through Modification of the Apical Polarity Protein CRUMBS2.PLoS genetics · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesHuman CAP10-like protein 46 kDa (hCLP46), also known as Poglut1, has been shown to be an essential regulator of Notch signalling. hCLP46 is overexpressed in primary acute myelogenous leukaemia, T-acute lymphoblastic leukaemia samples and other leukaemia cell lines. However, effects of hCLP46 overexpression, up to now, have remained unknown. MATERIALS AND
methodsIn this study, we established stable 293TRex cell lines inducibly overexpressing hCLP46, and knocked down hCLP6 with a specific small interfering RNA to explore function of the protein in Notch signalling and cell proliferation.
resultshCLP46 overexpression enhanced Notch1 activation in 293Trex cells in a ligand-dependent manner, with increased Notch signalling enhancing Hes1 expression. We further verified that overexpression of hCLP46 inhibited proliferation of 293TRexs and was correlated with increases in cyclin dependent kinase inhibitors p21 and p27, whereas reduced hCLP46 expression moderately increased cell proliferation. In addition, p21 and p27 protein levels were higher when Notch signalling was activated by EDTA treatment.
conclusionsTaken together, hCLP46 enhanced Notch activation and inhibited 293TRex cell proliferation through CDKI signalling.
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