ArticlePloS one2013
Elongated membrane tethers, individually anchored by high affinity α4β1/VCAM-1 complexes, are the quantal units of monocyte arrests.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Prediction-Based Algorithms for Long-Range High-Speed Force Spectroscopy.Journal of molecular recognition : JMR · 2026Article
- Nucleotide-Induced Nanoscale Changes in the Mechanical Properties of Rat Cerebellar Astrocytes: Selective Stimulation and Blocking of the Purinergic Receptor P2X7.International journal of molecular sciences · 2022Article
- Biomechanics of Neutrophil Tethers.Life (Basel, Switzerland) · 2021Review
- Article
- Hyaluronic acid selective anchoring to the cytoskeleton: An atomic force microscopy study.PloS one · 2018Article
- Monocyte Adhesion, Migration, and Extracellular Matrix Breakdown Are Regulated by Integrin αVβ3 inJournal of immunology (Baltimore, Md. : 1950) · 2017Article
- Reinforcement of integrin-mediated T-Lymphocyte adhesion by TNF-induced Inside-out Signaling.Scientific reports · 2016Article
- CD11c/CD18 Signals Very Late Antigen-4 Activation To Initiate Foamy Monocyte Recruitment during the Onset of Hypercholesterolemia.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Decoding Cytoskeleton-Anchored and Non-Anchored Receptors from Single-Cell Adhesion Force Data.Biophysical journal · 2015Article
- The NLRP3 Inflammasome Is a Pathogen Sensor for Invasive Entamoeba histolytica via Activation of α5β1 Integrin at the Macrophage-Amebae Intercellular Junction.PLoS pathogens · 2015Article
- Atomic force microscopy reveals a role for endothelial cell ICAM-1 expression in bladder cancer cell adherence.PloS one · 2014Article
- Agonist leukadherin-1 increases CD11b/CD18-dependent adhesion via membrane tethers.Biophysical journal · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The α4β1 integrin facilitates both monocyte rolling and adhesion to the vascular endothelium and is physiologically activated by monocyte chemoattractant protein (MCP-1). The current study investigated the initial events in the adhesion of THP-1 cells to immobilized Vascular Cell Adhesion Molecule 1 (VCAM-1). Using AFM force measurements, cell adhesion was shown to be mediated by two populations of α4β1/VCAM-1 complexes. A low affinity form of α4β1 was anchored to the elastic elements of the cytoskeleton, while a higher affinity conformer was coupled to the viscous elements of the cell membrane. Within 100 ms of contact, THP-1 cells, stimulated by co-immobilized MCP-1, exhibited a tremendous increase in adhesion to VCAM-1. Enhanced cell adhesion was accompanied by a local decoupling of the cell membrane from the cytoskeleton and the formation of long membrane tethers. The tethers were individually anchored by multiple α4β1/VCAM-1 complexes that prolonged the extension of the viscous tethers. In vivo, the formation of these membrane tethers may provide the quantal structural units for the arrest of rolling monocytes within the blood vessels.
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Registered trials
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