ArticlePloS one2013
Reducing uncertainty in within-host parameter estimates of influenza infection by measuring both infectious and total viral load.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Heterogeneous shedding of influenza by human subjects and its implications for epidemiology and control.Scientific reports · 2016Trial
- Analyzing Differences in Viral Dynamics Between Vaccinated and Unvaccinated RSV Patients.Epidemiologia (Basel, Switzerland) · 2025Article
- Spatial information allows inference of the prevalence of direct cell-to-cell viral infection.PLoS computational biology · 2024Article
- Heterogeneity of Rift Valley fever virus transmission potential across livestock hosts, quantified through a model-based analysis of host viral load and vector infection.PLoS computational biology · 2022Article
- Model Integration in Computational Biology: The Role of Reproducibility, Credibility and Utility.Frontiers in systems biology · 2022Article
- A mathematical model describing the localization and spread of influenza A virus infection within the human respiratory tract.PLoS computational biology · 2020Article
- Modeling Competitive Mixtures With the Lotka-Volterra Framework for More Complex Fitness Assessment Between Strains.Frontiers in microbiology · 2020Article
- Lung Aerosol Dynamics of Airborne Influenza A Virus-Laden Droplets and the Resultant Immune System Responses: An In Silico Study.Journal of aerosol science · 2019Article
- Characterization of Influenza B Virus Variants with Reduced Neuraminidase Inhibitor Susceptibility.Antimicrobial agents and chemotherapy · 2018Article
- Host-pathogen kinetics during influenza infection and coinfection: insights from predictive modeling.Immunological reviews · 2018Review
- A comparison of RSV and influenza in vitro kinetic parameters reveals differences in infecting time.PloS one · 2018Article
- The Mechanisms for Within-Host Influenza Virus Control Affect Model-Based Assessment and Prediction of Antiviral Treatment.Viruses · 2017Article
- Distinct immune responses and virus shedding in pigs following aerosol, intra-nasal and contact infection with pandemic swine influenza A virus, A(H1N1)09.Veterinary research · 2016Article
- Avian influenza viruses that cause highly virulent infections in humans exhibit distinct replicative properties in contrast to human H1N1 viruses.Scientific reports · 2016Article
- On the Role of CD8Frontiers in immunology · 2016Article
- Article
- Using Clinical Trial Simulators to Analyse the Sources of Variance in Clinical Trials of Novel Therapies for Acute Viral Infections.PloS one · 2016Article
- Review
- Innate Immunity and the Inter-exposure Interval Determine the Dynamics of Secondary Influenza Virus Infection and Explain Observed Viral Hierarchies.PLoS computational biology · 2015Article
- Assessing Uncertainty in A2 Respiratory Syncytial Virus Viral Dynamics.Computational and mathematical methods in medicine · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For in vivo studies of influenza dynamics where within-host measurements are fit with a mathematical model, infectivity assays (e.g. 50% tissue culture infectious dose; TCID50) are often used to estimate the infectious virion concentration over time. Less frequently, measurements of the total (infectious and non-infectious) viral particle concentration (obtained using real-time reverse transcription-polymerase chain reaction; rRT-PCR) have been used as an alternative to infectivity assays. We investigated the degree to which measuring both infectious (via TCID50) and total (via rRT-PCR) viral load allows within-host model parameters to be estimated with greater consistency and reduced uncertainty, compared with fitting to TCID50 data alone. We applied our models to viral load data from an experimental ferret infection study. Best-fit parameter estimates for the "dual-measurement" model are similar to those from the TCID50-only model, with greater consistency in best-fit estimates across different experiments, as well as reduced uncertainty in some parameter estimates. Our results also highlight how variation in TCID50 assay sensitivity and calibration may hinder model interpretation, as some parameter estimates systematically vary with known uncontrolled variations in the assay. Our techniques may aid in drawing stronger quantitative inferences from in vivo studies of influenza virus dynamics.
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