ArticlePloS one2013
Transcriptional analysis of temporal gene expression in germinating Clostridium difficile 630 endospores.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 70 citations in OpenAlex.
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- c-di-AMP signaling is required for bile salt resistance, osmotolerance, and long-term host colonization byScience signaling · 2022Article
- Imaging Clostridioides difficile Spore Germination and Germination Proteins.Journal of bacteriology · 2022Article
- Impact of Fungal Hyphae on Growth and Dispersal of Obligate Anaerobic Bacteria in Aerated Habitats.mBio · 2022Article
- A lipoprotein allosterically activates the CwlD amidase during Clostridioides difficile spore formation.PLoS genetics · 2021Article
- Levels and Characteristics of mRNAs in Spores of Firmicute Species.Journal of bacteriology · 2021Article
- Raman spectroscopy reveals alteration of spore compositions under different nutritional conditions in Lysinibacillus boronitolerans YS11.Journal of microbiology (Seoul, Korea) · 2021Article
- Structural, Metabolic and Evolutionary Comparison of Bacterial Endospore and Exospore Formation.Frontiers in microbiology · 2021Review
- The repertoire of ABC proteins inComputational and structural biotechnology journal · 2021Article
- Spore Germination of the Obligate BiotrophFrontiers in microbiology · 2021Article
- Peptidoglycan analysis reveals that synergistic deacetylase activity in vegetativeThe Journal of biological chemistry · 2020Article
- Integrative Analysis of Proteome and Transcriptome Dynamics during Bacillus subtilis Spore Revival.mSphere · 2020Article
- Review
- Bacterial Spore mRNA - What's Up With That?Frontiers in microbiology · 2020Review
- Vegetative Cell and Spore Proteomes ofJournal of proteome research · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Clostridium difficile is the leading cause of hospital acquired diarrhoea in industrialised countries. Under conditions that are not favourable for growth, the pathogen produces metabolically dormant endospores via asymmetric cell division. These are extremely resistant to both chemical and physical stress and provide the mechanism by which C. difficile can evade the potentially fatal consequences of exposure to heat, oxygen, alcohol, and certain disinfectants. Spores are the primary infective agent and must germinate to allow for vegetative cell growth and toxin production. While spore germination in Bacillus is well understood, little is known about C. difficile germination and outgrowth. Here we use genome-wide transcriptional analysis to elucidate the temporal gene expression patterns in C. difficile 630 endospore germination. We have optimized methods for large scale production and purification of spores. The germination characteristics of purified spores have been characterized and RNA extraction protocols have been optimized. Gene expression was highly dynamic during germination and outgrowth, and was found to involve a large number of genes. Using this genome-wide, microarray approach we have identified 511 genes that are significantly up- or down-regulated during C. difficile germination (p≤0.01). A number of functional groups of genes appeared to be co-regulated. These included transport, protein synthesis and secretion, motility and chemotaxis as well as cell wall biogenesis. These data give insight into how C. difficile re-establishes its metabolism, re-builds the basic structures of the vegetative cell and resumes growth.
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Registered trials
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