ArticlePloS one2013
Nitric oxide not apoptosis mediates differential killing of Mycobacterium bovis in bovine macrophages.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Immunopathogenesis of tuberculosis: cellular mechanisms and immune modulation.Veterinary research · 2025Review
- Modeling immune responses of cattle tomSphere · 2025Article
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- Intracellular behavior ofFrontiers in cellular and infection microbiology · 2023Article
- Genetically RelatedVeterinary sciences · 2019Article
- Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity.BMC veterinary research · 2019Article
- Article
- Endonuclease G takes part in AIF-mediated caspase-independent apoptosis in Mycobacterium bovis-infected bovine macrophages.Veterinary research · 2018Article
- Variation in the Early Host-Pathogen Interaction of Bovine Macrophages with Divergent Mycobacterium bovis Strains in the United Kingdom.Infection and immunity · 2018Article
- Divergent macrophage responses to Mycobacterium bovis among naturally exposed uninfected and infected cattle.Immunology and cell biology · 2017Article
- Badger macrophages fail to produce nitric oxide, a key anti-mycobacterial effector molecule.Scientific reports · 2017Article
- Nitric Oxide in the Pathogenesis and Treatment of Tuberculosis.Frontiers in microbiology · 2017Review
- Mycobacteria infect different cell types in the human lung and cause species dependent cellular changes in infected cells.BMC pulmonary medicine · 2016Article
- EBP50 induces apoptosis in macrophages by upregulating nitric oxide production to eliminate intracellular Mycobacterium tuberculosis.Scientific reports · 2016Article
- Requirement for endogenous heat shock factor 1 in inducible nitric oxide synthase induction in murine microglia.Journal of neuroinflammation · 2015Article
- Tuberculosis-resistant transgenic cattle.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
- Entry and elimination of marine mammal Brucella spp. by hooded seal (Cystophora cristata) alveolar macrophages in vitro.PloS one · 2013Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To identify the resistance phenotype against Mycobacterium bovis in cattle, we used a bactericidal assay that has been considered a marker of this trait. Three of 24 cows (12.5%) were phenotyped as resistant and 21 as susceptible. Resistance of bovine macrophages (MΦ) to BCG challenge was evaluated for its association with SLC11A1 GT microsatellite polymorphisms within 3'UTR region. Twenty-three cows (95.8%) had a GT13 genotype, reported as resistant, consequently the SLC11A1 polymorphism was not in agreement with our bactericidal assay results. MΦ of cows with resistant or susceptible phenotype were challenged in vitro with virulent M. bovis field strain or BCG, and nitric oxide production, bacterial killing and apoptosis induction were measured in resting and LPS-primed states. M. bovis field strain induced more apoptosis than BCG, although the difference was not significant. Resistant MΦ controlled better the replication of M. bovis (P<0.01), produced more nitric oxide (P<0.05) and were slightly more prone to undergo apoptosis than susceptible cells. LPS pretreatment of MΦ enhanced all the functional parameters analyzed. Inhibition of nitric oxide production with n (G)-monomethyl-L-arginine monoacetate enhanced replication of M. bovis but did not modify apoptosis rates in both resistant and susceptible MΦ. We conclude that nitric oxide production not apoptosis is a major determinant of macrophage resistance to M. bovis infection in cattle and that the influence of SLC11A1 gene 3'UTR polymorphism is not associated with this event.
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