ArticlePloS one2013
Epiblast ground state is controlled by canonical Wnt/β-catenin signaling in the postimplantation mouse embryo and epiblast stem cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
60 citing papers in PubMed, 107 citations in OpenAlex.
- PCBP1 orchestrates amino acid metabolism burst during the naïve-to-primed pluripotency transition.Stem cell reports · 2026Article
- NANOG is repurposed after implantation to repress Sox2 and begin pluripotency extinction.The EMBO journal · 2025Article
- Capture primed pluripotency in guinea pig.Stem cell reports · 2025Article
- A PLURIPOTENT STEM CELL PLATFORM FOR IN VITRO SYSTEMS GENETICS STUDIES OF MOUSE DEVELOPMENT.bioRxiv : the preprint server for biology · 2024Article
- Hallmarks of totipotent and pluripotent stem cell states.Cell stem cell · 2024Review
- Development of a chemically disclosed serum-free medium for mouse pluripotent stem cells.Frontiers in bioengineering and biotechnology · 2024Article
- Epigenetic dynamics during capacitation of naïve human pluripotent stem cells.Science advances · 2023Article
- Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells.Cell reports methods · 2023Article
- Rat epiblast-derived stem cells recapitulate the attributes of pre-gastrulation epiblast.Cell reports methods · 2023Article
- Population-level antagonism between FGF and BMP signaling steers mesoderm differentiation in embryonic stem cells.Biology open · 2023Article
- Macroscopic quorum sensing sustains differentiating embryonic stem cells.Nature chemical biology · 2023Article
- Esrrb guides naive pluripotent cells through the formative transcriptional programme.Nature cell biology · 2023Article
- Wnt signal-dependent antero-posterior specification of early-stage CNS primordia modeled in EpiSC-derived neural stem cells.Frontiers in cell and developmental biology · 2023Article
- Suppression of YAP safeguards human naïve pluripotency.Development (Cambridge, England) · 2022Article
- Laminin111-based defined culture promoting self-renewing human pluripotent stem cells with properties of the early post-implantation epiblast.Stem cell reports · 2022Article
- Rapid and robust directed differentiation of mouse epiblast stem cells into definitive endoderm and forebrain organoids.Development (Cambridge, England) · 2022Article
- Stepwise pluripotency transitions in mouse stem cells.EMBO reports · 2022Review
- 8C-like cells capture the human zygotic genome activation program in vitro.Cell stem cell · 2022Article
- β-catenin perturbations control differentiation programs in mouse embryonic stem cells.iScience · 2022Article
- Intermittent ERK oscillations downstream of FGF in mouse embryonic stem cells.Development (Cambridge, England) · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epiblast stem cells (EpiSCs) are primed pluripotent stem cells and can be derived from postimplantation mouse embryos. We now show that the absence of canonical Wnt/β-catenin signaling is essential for maintenance of the undifferentiated state in mouse EpiSCs and in the epiblast of mouse embryos. Attenuation of Wnt signaling with the small-molecule inhibitor XAV939 or deletion of the β-catenin gene blocked spontaneous differentiation of EpiSCs toward mesoderm and enhanced the expression of pluripotency factor genes, allowing propagation of EpiSCs as a homogeneous population. EpiSCs were efficiently established and propagated from single epiblast cells in the presence of both XAV939 and the Rho kinase (ROCK) inhibitor Y27632. Cell transplantation revealed that EpiSCs were able to contribute to primordial germ cells and descendants of all three germ layers in a host embryo, suggesting that they maintained pluripotency, even after prolonged culture with XAV939. Such an improvement in the homogeneity of pluripotency achieved with the use of a Wnt inhibitor should prove advantageous for manipulation of primed pluripotent stem cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.