ArticlePloS one2013
Characterization of cell wall lipids from the pathogenic phase of Paracoccidioides brasiliensis cultivated in the presence or absence of human plasma.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Comparative microbial lipidomics: structural diversity, adaptive strategies, and analytical advances.Folia microbiologica · 2026Review
- Characterizing extracellular vesicles of human fungal pathogens.Nature microbiology · 2025Review
- Identification and immunogenic potential of glycosylphosphatidylinositol-anchored proteins inFrontiers in fungal biology · 2023Article
- Current Status on Extracellular Vesicles from the Dimorphic Pathogenic Species of Paracoccidioides.Current topics in microbiology and immunology · 2021Article
- Lipid Secretion by Parasitic Cells ofFrontiers in cellular and infection microbiology · 2021Article
- Analysis ofFrontiers in cellular and infection microbiology · 2020Article
- Monohexosylceramides fromMolecules (Basel, Switzerland) · 2018Article
- The Interaction of Human Pathogenic Fungi With C-Type Lectin Receptors.Frontiers in immunology · 2018Review
- Identification of membrane proteome ofFuture science OA · 2017Article
- Antibodies Against Glycolipids Enhance Antifungal Activity of Macrophages and Reduce Fungal Burden After Infection with Paracoccidioides brasiliensis.Frontiers in microbiology · 2016Article
- Paracoccidioides brasiliensis AND Paracoccidioides lutzii, A SECRET LOVE AFFAIR.Revista do Instituto de Medicina Tropical de Sao Paulo · 2015Review
- Challenges posed by extracellular vesicles from eukaryotic microbes.Current opinion in microbiology · 2014Review
- Glycolipid sensing and innate immunity in paracoccidioidomycosis.Mycopathologia · 2014Article
- Structural diversity and biological significance of glycosphingolipids in pathogenic and opportunistic fungi.Frontiers in cellular and infection microbiology · 2014Review
- Characterization of Lipids and Proteins Associated to the Cell Wall of the Acapsular Mutant Cryptococcus neoformans Cap 67.The Journal of eukaryotic microbiologyArticle
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundThe fungal cell wall is a complex and dynamic outer structure. In pathogenic fungi its components interact with the host, determining the infection fate. The present work aimed to characterize cell wall lipids from P. brasiliensis grown in the presence and absence of human plasma. We compared the results from isolates Pb3 and Pb18, which represent different phylogenetic species that evoke distinct patterns of experimental paracoccidioidomycosis. METHODOLOGY/PRINCIPAL
findingsWe comparatively characterized cell wall phospholipids, fatty acids, sterols, and neutral glycolipids by using both electrospray ionization- and gas chromatography-mass spectrometry analyses of lipids extracted with organic solvents followed by fractionation in silica-gel-60. We detected 49 phospholipid species in Pb3 and 38 in Pb18, including phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylglycerol, phosphatidylinositol, and phosphatidic acid. In both Pb3 and Pb18, PC and PE had the most numerous species. Among the fatty acids, C18:1 and C18:2 were the most abundant species in both isolates, although C18:2 was more abundant in Pb18. There was a different effect of plasma supplementation on fatty acids depending on the fungal isolate. The prevalent glycolipid species was Hex-C18:0-OH/d19:2-Cer, although other four minor species were also detected. The most abundant sterol in all samples was brassicasterol. Distinct profiles of cell wall and total yeast sterols suggested that the preparations were enriched for cell wall components. The presence of plasma in the culture medium specially increased cell wall brassicasterol abundance and also other lipids. CONCLUSIONS/SIGNIFICANCE: We here report an original comparative lipidomic analysis of P. brasiliensis cell wall. Our results open doors to understanding the role of cell wall lipids in fungal biology, and interaction with anti-fungal drugs and the host.
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