ArticlePloS one2013
Systems analysis of lactose metabolism in Trichoderma reesei identifies a lactose permease that is essential for cellulase induction.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 142 citations in OpenAlex.
- Reducing sugars: a potential factor for onion plant salinity adaptation.BMC plant biology · 2025Article
- Advances in fungal sugar transporters: unlocking the potential of second-generation bioethanol production.Applied microbiology and biotechnology · 2025Review
- Expansion of maltose/sucrose related transporters in Ascomycetes and their association with corresponding disaccharide utilization.Current research in microbial sciences · 2025Article
- Correlating sugar transporter expression and activities to identify transporters for an orphan sugar substrate.Applied microbiology and biotechnology · 2024Article
- Developing endophytic Penicillium oxalicum as a source of lignocellulolytic enzymes for enhanced hydrolysis of biorefinery relevant pretreated rice straw.Bioprocess and biosystems engineering · 2024Article
- Transgressive phenotypes from outbreeding between theMicrobiology spectrum · 2024Article
- Unveiling a Microexon Switch: Novel Regulation of the Activities of Sugar Assimilation and Plant-Cell-Wall-Degrading Xylanases and Cellulases by Xlr2 inInternational journal of molecular sciences · 2024Article
- The transcription factor STE12 influences growth on several carbon sources and production of dehydroacetic acid (DHAA) in Trichoderma reesei.Scientific reports · 2024Article
- CRISPR/Cas9 mediated gene editing of transcription factor ACE1 for enhanced cellulase production in thermophilic fungus Rasamsonia emersonii.Fungal biology and biotechnology · 2023Article
- Intracellular Sugar Transporters Facilitate Cellulase Synthesis inBiomolecules · 2023Article
- Discovery of ER-localized sugar transporters for cellulase production with lac1 being essential.Biotechnology for biofuels and bioproducts · 2022Article
- Inducer-free cellulase production system based on the constitutive expression of mutated XYR1 and ACE3 in the industrial fungus Trichoderma reesei.Scientific reports · 2022Article
- Functional Characterization of Sugar Transporter CRT1 Reveals Differential Roles of Its C-Terminal Region in Sugar Transport and Cellulase Induction in Trichoderma reesei.Microbiology spectrum · 2022Article
- Induction of cellulase production inRSC advances · 2022Article
- Press water from the mechanical drying of Douglas-fir wood chips has multiple beneficial effects on lignocellulolytic fungi.Fungal biology and biotechnology · 2022Article
- Transmembrane transport process and endoplasmic reticulum function facilitate the role of gene cel1b in cellulase production of Trichoderma reesei.Microbial cell factories · 2022Article
- Factors regulating cellulolytic gene expression in filamentous fungi: an overview.Microbial cell factories · 2022Review
- Simultaneous manipulation of multiple genes within a same regulatory stage for iterative evolution of Trichoderma reesei.Biotechnology for biofuels and bioproducts · 2022Article
- Assessing the intracellular primary metabolic profile ofFrontiers in fungal biology · 2022Article
- Elimination of the Sugar Transporter GAT1 Increased Xylanase I Production inFrontiers in microbiology · 2022Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Trichoderma reesei colonizes predecayed wood in nature and metabolizes cellulose and hemicellulose from the plant biomass. The respective enzymes are industrially produced for application in the biofuel and biorefinery industry. However, these enzymes are also induced in the presence of lactose (1,4-0-ß-d-galactopyranosyl-d-glucose), a waste product from cheese manufacture or whey processing industries. In fact, lactose is the only soluble carbon source that induces these enzymes in T. reesei on an industrial level but the reason for this unique phenomenon is not understood. To answer this question, we used systems analysis of the T. reesei transcriptome during utilization of lactose. We found that the respective CAZome encoded all glycosyl hydrolases necessary for cellulose degradation and particularly for the attack of monocotyledon xyloglucan, from which ß-galactosides could be released that may act as the inducers of T. reesei's cellulases and hemicellulases. In addition, lactose also induces a high number of putative transporters of the major facilitator superfamily. Deletion of fourteen of them identified one gene that is essential for lactose utilization and lactose uptake, and for cellulase induction by lactose (but not sophorose) in pregrown mycelia of T. reesei. These data shed new light on the mechanism by which T. reesei metabolizes lactose and offers strategies for its improvement. They also illuminate the key role of ß-D-galactosides in habitat specificity of this fungus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.