ArticlePloS one2013
The human gut chip "HuGChip", an explorative phylogenetic microarray for determining gut microbiome diversity at family level.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 51 citations in OpenAlex.
- Gut-on-a-chip: Current progress and future opportunities.Biomaterials · 2020Review
- The Simplified Human Intestinal Microbiota (SIHUMIx) Shows High Structural and Functional Resistance against Changing Transit Times inMicroorganisms · 2019Article
- Species Level Description of the Human Ileal Bacterial Microbiota.Scientific reports · 2018Article
- FibroChip, a Functional DNA Microarray to Monitor Cellulolytic and Hemicellulolytic Activities of Rumen Microbiota.Frontiers in microbiology · 2018Article
- Intestinal microbiome landscaping: insight in community assemblage and implications for microbial modulation strategies.FEMS microbiology reviews · 2017Review
- Colonic Transit Time Is a Driven Force of the Gut Microbiota Composition and Metabolism: In Vitro Evidence.Journal of neurogastroenterology and motility · 2017Article
- Functional amplification and preservation of human gut microbiota.Microbial ecology in health and disease · 2017Article
- Factoring the intestinal microbiome into the pathogenesis of autoimmune hepatitis.World journal of gastroenterology · 2016Review
- Trimethylamine and Trimethylamine N-Oxide, a Flavin-Containing Monooxygenase 3 (FMO3)-Mediated Host-Microbiome Metabolic Axis Implicated in Health and Disease.Drug metabolism and disposition: the biological fate of chemicals · 2016Review
- An Introduction to Programming for Bioscientists: A Python-Based Primer.PLoS computational biology · 2016Article
- Whole Rye Consumption Improves Blood and Liver n-3 Fatty Acid Profile and Gut Microbiota Composition in Rats.PloS one · 2016Article
- In Silico Genomic Fingerprints of the Bacillus anthracis Group Obtained by Virtual Hybridization.Microarrays (Basel, Switzerland) · 2015Article
- Archaea and the human gut: new beginning of an old story.World journal of gastroenterology · 2014Review
- Article
- The human intestinal microbiome at extreme ages of life. Dietary intervention as a way to counteract alterations.Frontiers in genetics · 2014Review
Corrections and comments
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Authors and funding
15 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Evaluating the composition of the human gut microbiota greatly facilitates studies on its role in human pathophysiology, and is heavily reliant on culture-independent molecular methods. A microarray designated the Human Gut Chip (HuGChip) was developed to analyze and compare human gut microbiota samples. The PhylArray software was used to design specific and sensitive probes. The DNA chip was composed of 4,441 probes (2,442 specific and 1,919 explorative probes) targeting 66 bacterial families. A mock community composed of 16S rRNA gene sequences from intestinal species was used to define the threshold criteria to be used to analyze complex samples. This was then experimentally verified with three human faecal samples and results were compared (i) with pyrosequencing of the V4 hypervariable region of the 16S rRNA gene, (ii) metagenomic data, and (iii) qPCR analysis of three phyla. When compared at both the phylum and the family level, high Pearson's correlation coefficients were obtained between data from all methods. The HuGChip development and validation showed that it is not only able to assess the known human gut microbiota but could also detect unknown species with the explorative probes to reveal the large number of bacterial sequences not yet described in the human gut microbiota, overcoming the main inconvenience encountered when developing microarrays.
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