ArticleReproduction (Cambridge, England)2013
Isolation, genetic manipulation, and transplantation of canine spermatogonial stem cells: progress toward transgenesis through the male germ-line.
Article in Reproduction (Cambridge, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Retinoic Acid Signaling in Male Reproductive Biology: From Germ Cell Regulation to Contraceptive Innovation Within a One Health Framework.Animals : an open access journal from MDPI · 2026Review
- Male germ cell technologies in large animals.The Journal of reproduction and development · 2026Review
- Spermatogonial Stem Cells in Domestic Animals: Current Insights and Future Directions with a Focus on Dogs.Veterinary sciences · 2025Review
- Transplantation of spermatogonial stem cells in stallions.Journal of animal science and technology · 2024Review
- Optimizing Immature Testicular Tissue and Cell Transplantation Results: Comparing Transplantation Sites and Scaffolds.International journal of fertility & sterility · 2023Review
- Porcine Germ Cells Phenotype during Embryonic and Adult Development.Animals : an open access journal from MDPI · 2023Article
- Detection of spermatogonial stem cells in testicular tissue of dogs with chronic asymptomatic orchitis.Frontiers in veterinary science · 2023Article
- Sertoli, Leydig, and Spermatogonial Cells' Specific Gene and Protein Expressions as Dog Testes Evolve from Immature into Mature States.Animals : an open access journal from MDPI · 2022Article
- Article
- Spermatogonial Stem Cell Transplantation in Large Animals.Animals : an open access journal from MDPI · 2021Review
- Step by Step about Germ Cells Development in Canine.Animals : an open access journal from MDPI · 2021Review
- Xenotransplantation of canine spermatogonial stem cells (cSSCs) regulated by FSH promotes spermatogenesis in infertile mice.Stem cell research & therapy · 2019Article
- Stem cells on regenerative and reproductive science in domestic animals.Veterinary research communications · 2019Review
- Spermatogonial stem cells.Biology of reproduction · 2018Review
- Establishment of a surgically induced cryptorchidism canine recipient model for spermatogonial stem cell transplantation.Laboratory animal research · 2016Article
- Isolation and Identification of Prepubertal Buffalo (Bubalus bubalis) Spermatogonial Stem Cells.Asian-Australasian journal of animal sciences · 2016Article
- Spermatogonial stem cells: Current biotechnological advances in reproduction and regenerative medicine.World journal of stem cells · 2015Article
- Beyond the mouse monopoly: studying the male germ line in domestic animal models.ILAR journal · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The dog is recognized as a highly predictive model for preclinical research. Its size, life span, physiology, and genetics more closely match human parameters than do those of the mouse model. Investigations of the genetic basis of disease and of new regenerative treatments have frequently taken advantage of canine models. However, full utility of this model has not been realized because of the lack of easy transgenesis. Blastocyst-mediated transgenic technology developed in mice has been very slow to translate to larger animals, and somatic cell nuclear transfer remains technically challenging, expensive, and low yield. Spermatogonial stem cell (SSC) transplantation, which does not involve manipulation of ova or blastocysts, has proven to be an effective alternative approach for generating transgenic offspring in rodents and in some large animals. Our recent demonstration that canine testis cells can engraft in a host testis, and generate donor-derived sperm, suggests that SSC transplantation may offer a similar avenue to transgenesis in the canine model. Here, we explore the potential of SSC transplantation in dogs as a means of generating canine transgenic models for preclinical models of genetic diseases. Specifically, we i) established markers for identification and tracking canine spermatogonial cells; ii) established methods for enrichment and genetic manipulation of these cells; iii) described their behavior in culture; and iv) demonstrated engraftment of genetically manipulated SSC and production of transgenic sperm. These findings help to set the stage for generation of transgenic canine models via SSC transplantation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.