ArticleProceedings of the National Academy of Sciences of the United States of America2013
Structural insights into the N-terminal GIY-YIG endonuclease activity of Arabidopsis glutaredoxin AtGRXS16 in chloroplasts.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Emerging roles of glutaredoxins in plant metabolism.Journal of experimental botany · 2026Review
- Unveiling a novel broad-host-range cyanomyovirus cross-infecting Prochlorococcus and Synechococcus.BMC biology · 2025Article
- Genome-wide identification and expression analysis of glutaredoxin in Puccinellia tenuiflora under salinity stress.BMC plant biology · 2025Article
- Holliday junction resolution by At-HIGLE: an SLX1 lineage endonuclease from Arabidopsis thaliana with a novel in-built regulatory mechanism.Nucleic acids research · 2022Article
- Identification and Function of Apicoplast Glutaredoxins inInternational journal of molecular sciences · 2021Article
- Article
- Plasticity in plastid redox networks: evolution of glutathione-dependent redox cascades and glutathionylation sites.BMC plant biology · 2021Article
- Iron-sulfur cluster biogenesis, trafficking, and signaling: Roles for CGFS glutaredoxins and BolA proteins.Biochimica et biophysica acta. Molecular cell research · 2021Review
- Redox Modification of the Iron-Sulfur Glutaredoxin GRXS17 Activates Holdase Activity and Protects Plants from Heat Stress.Plant physiology · 2020Article
- Development of a rapid and efficient protoplast isolation and transfection method for chickpea (MethodsX · 2020Article
- Mining for protein S-sulfenylation inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- EFFECTOR OF TRANSCRIPTION factors are novel plant-specific regulators associated with genomic DNA methylation in Arabidopsis.The New phytologist · 2019Article
- Reactive Oxygen Species and the Redox-Regulatory Network in Cold Stress Acclimation.Antioxidants (Basel, Switzerland) · 2018Review
- Assembly and Transfer of Iron-Sulfur Clusters in the Plastid.Frontiers in plant science · 2018Review
- Arabidopsis Glutaredoxin S17 Contributes to Vegetative Growth, Mineral Accumulation, and Redox Balance during Iron Deficiency.Frontiers in plant science · 2017Article
- Protein networks identify novel symbiogenetic genes resulting from plastid endosymbiosis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Characterization of poplar GrxS14 in different structural forms.Protein & cell · 2014Article
- Glutaredoxins are essential for stress adaptation in the cyanobacterium Synechocystis sp. PCC 6803.Frontiers in plant science · 2013Article
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glutaredoxins (Grxs) have been identified across taxa as important mediators in various physiological functions. A chloroplastic monothiol glutaredoxin, AtGRXS16 from Arabidopsis thaliana, comprises two distinct functional domains, an N-terminal domain (NTD) with GlyIleTyr-TyrIleGly (GIY-YIG) endonuclease motif and a C-terminal Grx module, to coordinate redox regulation and DNA cleavage in chloroplasts. Structural determination of AtGRXS16-NTD showed that it possesses a GIY-YIG endonuclease fold, but the critical residues for the nuclease activity are different from typical GIY-YIG endonucleases. AtGRXS16-NTD was able to cleave λDNA and chloroplast genomic DNA, and the nuclease activity was significantly reduced in AtGRXS16. Functional analysis indicated that AtGRXS16-NTD could inhibit the ability of AtGRXS16 to suppress the sensitivity of yeast grx5 cells to oxidative stress; however, the C-terminal Grx domain itself and AtGRXS16 with a Cys123Ser mutation were active in these cells and able to functionally complement a Grx5 deficiency in yeast. Furthermore, the two functional domains were shown to be negatively regulated through the formation of an intramolecular disulfide bond. These findings unravel a manner of regulation for Grxs and provide insights into the mechanistic link between redox regulation and DNA metabolism in chloroplasts.
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