ArticleInfection and immunity2013
Manganese and zinc regulate virulence determinants in Borrelia burgdorferi.
Article in Infection and immunity, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 53 citations in OpenAlex.
- The Dual Role of Zinc Homeostasis in Gram-Negative Bacterial Pathogenicity and Host Immune Defense.Microorganisms · 2026Review
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- Lyme borreliosis.Nature reviews. Disease primers · 2026Review
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- Functional investigation of Zur in metal ion homeostasis, motility and multiple stresses resistance in cyanobacteria Synechocystis sp. PCC 6803.Stress biology · 2025Article
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- CspZ variant-specific interaction with factor H incorporates a metal site to support Lyme borreliae complement evasion.The Journal of biological chemistry · 2025Article
- The Leptospira interrogans proteome's response to zinc highlights the potential involvement of this metal in translational-machinery and virulence.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2024Article
- Positive feedback regulation between RpoS and BosR in the Lyme disease pathogen.bioRxiv : the preprint server for biology · 2024Article
- Assessment of the hypothetical protein BB0616 in the murine infection ofInfection and immunity · 2024Article
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- Effect of zinc source (zinc sulfate or zinc hydroxychloride) on relative abundance of fecalJDS communications · 2022Article
- The Consistent Tick-Vertebrate Infectious Cycle of the Lyme Disease Spirochete Enables Borrelia burgdorferi To Control Protein Expression by Monitoring Its Physiological Status.Journal of bacteriology · 2022Review
- The peptidoglycan-associated protein NapA plays an important role in the envelope integrity and in the pathogenesis of the lyme disease spirochete.PLoS pathogens · 2021Article
- Lyme Disease Pathogenesis.Current issues in molecular biology · 2021Review
- Gene Regulation and Transcriptomics.Current issues in molecular biology · 2021Article
- Innate Immune Memory to RepeatedJournal of immunology (Baltimore, Md. : 1950) · 2020Article
- "Repurposing" Disulfiram in the Treatment of Lyme Disease and Babesiosis: Retrospective Review of First 3 Years' Experience in One Medical Practice.Antibiotics (Basel, Switzerland) · 2020Article
- AttenuatedVaccines · 2020Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Borrelia burgdorferi, the causative agent of Lyme disease, must adapt to two diverse niches, an arthropod vector and a mammalian host. RpoS, an alternative sigma factor, plays a central role in spirochetal adaptation to the mammalian host by governing expression of many genes important for mammalian infection. B. burgdorferi is known to be unique in metal utilization, and little is known of the role of biologically available metals in B. burgdorferi. Here, we identified two transition metal ions, manganese (Mn(2+)) and zinc (Zn(2+)), that influenced regulation of RpoS. The intracellular Mn(2+) level fluctuated approximately 20-fold under different conditions and inversely correlated with levels of RpoS and the major virulence factor OspC. Furthermore, an increase in intracellular Mn(2+) repressed temperature-dependent induction of RpoS and OspC; this repression was overcome by an excess of Zn(2+). Conversely, a decrease of intracellular Mn(2+) by deletion of the Mn(2+) transporter gene, bmtA, resulted in elevated levels of RpoS and OspC. Mn(2+) affected RpoS through BosR, a Fur family homolog that is required for rpoS expression: elevated intracellular Mn(2+) levels greatly reduced the level of BosR protein but not the level of bosR mRNA. Thus, Mn(2+) and Zn(2+) appeared to be important in modulation of the RpoS pathway that is essential to the life cycle of the Lyme disease spirochete. This finding supports the emerging notion that transition metals such as Mn(2+) and Zn(2+) play a critical role in regulation of virulence in bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.