ArticlePlant molecular biology2013
Identification of key amino acids for the evolution of promoter target specificity of anthocyanin and proanthocyanidin regulating MYB factors.
Article in Plant molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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60 citing papers in PubMed, 119 citations in OpenAlex.
- Proanthocyanidins: structure, biosynthesis, regulation, and structure-activity relationships.aBIOTECH · 2026Review
- Integrated Metabolome-Transcriptome Profiling Identifies JrMYB8 as a Repressor of Polyphenol Biosynthesis in Walnut (Plants (Basel, Switzerland) · 2026Article
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- Haplotype-resolved genome assembly provides new insights into the genomic origin of purple colour in Prunus mume.Plant biotechnology journal · 2025Article
- Screening of MYB1R1 interaction withPeerJ · 2025Article
- The R2R3 MYB Ruby1 is activated by two cold responsive ethylene response factors, via the retrotransposon in its promoter, to positively regulate anthocyanin biosynthesis in citrus.The Plant journal : for cell and molecular biology · 2024Article
- Identification of a candidate gene for the I locus determining the dominant white bulb color in onion (Allium cepa L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Promoter replication of grape MYB transcription factor is associated with a new red flesh phenotype.Plant cell reports · 2024Article
- A near-complete assembly of asparagus bean provides insights into anthocyanin accumulation in pods.Plant biotechnology journal · 2023Article
- Article
- MYB Transcription FactorInternational journal of molecular sciences · 2023Article
- Novel R2R3 MYB transcription factors regulate anthocyanin synthesis in Aubergine tomato plants.BMC plant biology · 2023Article
- Co-expression network analyses of anthocyanin biosynthesis genes in Ruellia (Wild Petunias; Acanthaceae).BMC ecology and evolution · 2022Article
- Expression of apple MdMYB10 transcription factor in sugar beet with a screenable marker role and antimicrobial activity.3 Biotech · 2022Article
- EutremaFrontiers in plant science · 2022Article
- An LTR retrotransposon in the promoter of aHorticulture research · 2022Article
- Glucose Supply Induces PsMYB2-Mediated Anthocyanin Accumulation inFrontiers in plant science · 2022Article
- Anthocyanin synthesis potential in betalain-producing Caryophyllales plants.Journal of plant research · 2021Article
- Light Induced Regulation Pathway of Anthocyanin Biosynthesis in Plants.International journal of molecular sciences · 2021Review
- Genome-Wide Mining of MYB Transcription Factors in the Anthocyanin Biosynthesis Pathway of Gossypium Hirsutum.Biochemical genetics · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A complex of R2R3-MYB and bHLH transcription factors, stabilized by WD40 repeat proteins, regulates gene transcription for plant cell pigmentation and epidermal cell morphology. It is the MYB component of this complex which specifies promoter target activation. The Arabidopsis MYB TT2 regulates proanthocyanidin (PA) biosynthesis by activating the expression of ANR (anthocyanidin reductase), the gene product of which catalyzes the first committed step of this pathway. Conversely the closely related MYB PAP4 (AtMYB114) regulates the anthocyanin pathway and specifically activates UFGT (UDP-glucose:flavonoid-3-O-glucosyltransferase), encoding the first enzyme of the anthocyanin pathway. Both at the level of structural and regulatory genes, evolution of PA biosynthesis proceeded anthocyanin biosynthesis and we have identified key residues in these MYB transcription factors for the evolution of target promoter specificity. Using chimeric and point mutated variants of TT2 and PAP4 we found that exchange of a single amino acid, Gly/Arg(39) in the R2 domain combined with an exchange of a four amino acid motif in the R3 domain, could swap the pathway selection of TT2 and PAP4, thereby converting in planta specificity of the PA towards the anthocyanin pathway and vice versa. The general importance of these amino acids for target specificity was also shown for the grapevine transcription factors VvMYBPA2 and VvMYBA2 which regulate PAs and anthocyanins, respectively. These results provide an insight into the evolution of the different flavonoid regulators from a common ancestral gene.
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