ArticleJournal of biomolecular NMR2013
Solid-state NMR enhanced by dynamic nuclear polarization as a novel tool for ribosome structural biology.
Article in Journal of biomolecular NMR, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- DNP enhanced solid-state NMR of lattice-like microcrystalline protein assemblies facilitated by co-assembly with dinitroxide-tagged proteins.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2026Article
- EPR spectroscopy in the study of ribosomal complexes.Biophysical reviews · 2025Review
- Cysteine oxidation and disulfide formation in the ribosomal exit tunnel.Nature communications · 2020Article
- High-sensitivity protein solid-state NMR spectroscopy.Current opinion in structural biology · 2019Review
- Frequency-Swept Integrated and Stretched Solid Effect Dynamic Nuclear Polarization.The journal of physical chemistry letters · 2018Article
- How Messenger RNA and Nascent Chain Sequences Regulate Translation Elongation.Annual review of biochemistry · 2018Review
- Peptide and Protein Dynamics and Low-Temperature/DNP Magic Angle Spinning NMR.The journal of physical chemistry. B · 2017Article
- On the use of ultracentrifugal devices for routine sample preparation in biomolecular magic-angle-spinning NMR.Journal of biomolecular NMR · 2017Article
- Gd(iii) and Mn(ii) complexes for dynamic nuclear polarization: small molecular chelate polarizing agents and applications with site-directed spin labeling of proteins.Physical chemistry chemical physics : PCCP · 2016Article
- Structural analysis of a signal peptide inside the ribosome tunnel by DNP MAS NMR.Science advances · 2016Article
- Sensitive proton-detected solid-state NMR spectroscopy of large proteins with selective CH3 labelling: application to the 50S ribosome subunit.Chemical communications (Cambridge, England) · 2016Article
- Intermolecular Interactions and Protein Dynamics by Solid-State NMR Spectroscopy.Angewandte Chemie (International ed. in English) · 2015Article
- Intermolecular Interactions and Protein Dynamics by Solid-State NMR Spectroscopy.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2015Article
- Mechanisms of dynamic nuclear polarization in insulating solids.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2015Review
- NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field.Journal of biomolecular NMR · 2014Article
- Rapid proton-detected NMR assignment for proteins with fast magic angle spinning.Journal of the American Chemical Society · 2014Article
- Dynamic nuclear polarization of (1)H, (13)C, and (59)Co in a tris(ethylenediamine)cobalt(III) crystalline lattice doped with Cr(III).Journal of the American Chemical Society · 2014Article
- Synthesis and evaluation of nitroxide-based oligoradicals for low-temperature dynamic nuclear polarization in solid state NMR.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2014Article
- Perturbation of nuclear spin polarizations in solid state NMR of nitroxide-doped samples by magic-angle spinning without microwaves.The Journal of chemical physics · 2014Article
- Dynamic nuclear polarization NMR enables the analysis of Sn-Beta zeolite prepared with natural abundance ¹¹⁹Sn precursors.Journal of the American Chemical Society · 2014Article
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9 authors.
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Abstract
The impact of Nuclear Magnetic Resonance (NMR) on studies of large macromolecular complexes hinges on improvements in sensitivity and resolution. Dynamic nuclear polarization (DNP) in the solid state can offer improved sensitivity, provided sample preparation is optimized to preserve spectral resolution. For a few nanomoles of intact ribosomes and an 800 kDa ribosomal complex we demonstrate that the combination of DNP and magic-angle spinning NMR (MAS-NMR) allows one to overcome current sensitivity limitations so that homo- and heteronuclear (13)C and (15)N NMR correlation spectra can be recorded. Ribosome particles, directly pelleted and frozen into an NMR rotor, yield DNP signal enhancements on the order of ~25-fold and spectra that exhibit narrow linewidths, suitable for obtaining site-specific information. We anticipate that the same approach is applicable to other high molecular weight complexes.
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